'Isocitrate dehydrogenase' presentation slideshows

Citric Acid Cycle

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Chapter 19

Chapter 19. The Tricarboxylic Acid Cycle Biochemistry by Reginald Garrett and Charles Grisham. Essential Question. How is pyruvate oxidized under aerobic conditions Under aerobic conditions, pyruvate is converted to acetyl-CoA and oxidized to CO 2 in the TCA cycle

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12.3 The Citric Acid Cycle Oxidizes AcetylCoA

12.3 The Citric Acid Cycle Oxidizes AcetylCoA. Table 12.2. Summary of the citric acid cycle. For each acetyl CoA which enters the cycle: (1) Two molecules of CO 2 are released (2) Coenzymes NAD + and Q are reduced (3) One GDP (or ADP) is phosphorylated

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Lecture 7

Lecture 7. August 15, 2005 Lehninger (4 th Edition), Chapter 16 Oxidative phosphorylation: Chapter 19. Products from one turn of the Krebs cycle. LNC Fig.16.13. the only membrane- bound enzyme in the TCA cycle. LNC Fig.16.7. THE GLYOXYLATE CYCLE. LNC Chapter 16, pages 623-626.

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Lecture 26

Lecture 26. TCA cycle. Page 766. Figure 21-17b Factors controlling the activity of the PDC. ( b ) Covalent modification in the eukaryotic complex. Page 781. Control by phosporylation/dephosphorylation. Occurs only in eukaryotic complexes

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Engineering of Biological Processes Lecture 6: Modeling metabolism

Engineering of Biological Processes Lecture 6: Modeling metabolism. Mark Riley, Associate Professor Department of Ag and Biosystems Engineering The University of Arizona, Tucson, AZ 2007. Objectives: Lecture 6. Model metabolic reactions to shift carbon and resources down certain paths

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Lecture 30

Lecture 30. Pyruvate Oxidation and the Citric Acid Cycle. Aerobic Fate of Pyruvate: Overview. Mitochondria: Site of Oxidations. Pyruvate Oxidation to Acetyl-CoA. Pyruvate Dehydrogenase Complex. Complex enzyme 60 polypeptides of 3 kinds In mitocondrion matrix Regulated

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Stages of Metabolism

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Chapt. 20 TCA cycle

Chapt. 20 TCA cycle. Ch. 20 Tricarboxylic acid cyle Student Learning Outcomes: Describe relevance of TCA cycle Acetyl CoA funnels products Describe reactions of TCA cycle in cell respiration: 2C added, oxidations, rearrangements-> NADH, FAD(2H), GTP, CO 2 produced

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Citric Acid Cycle

Citric Acid Cycle. What is it? Series of rxns that oxidize acetyl CoA to 2CO 2 in a manner that conserves the liberated free energy for ATP production Breakdown products of glc, fats and amino acids are all oxidized via the cycle

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Citric Acid Cycle Generates Reductive Equivalents Utilized in Respiration

Citric Acid Cycle Generates Reductive Equivalents Utilized in Respiration. Citric Acid Cycle: A Two Stage Process. Stage One : C 4 + C 2 condensation C 6 tricarboxylic acid Oxidative decarboxylation 2 CO 2 Succinyl CoA Net Result: 2NADH + 2CO 2 + Succinyl CoA.

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Citric Acid Cycle 2

Citric Acid Cycle 2. C483 Spring 2013. 1. A metabolic pathway that is involved in both energy production and biosynthesis is Anaplerotic amphibolic C) Duplibolic Cataplerotic

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ENERGY YIELD

4. Oxaloacetate. 6. Citrate. 4. Malate. Isocitrate. 6. CH 3 C. S-CoA. ~. O. CO 2. CO 2. 4. Fumarate. 5. a -ketoglutarate. 4. 4. Succinate. Succinyl-CoA. ENERGY YIELD. NADH + H +. 3 ATP. 3 ATP. 1 ATP. 2 ATP. NADH + H +. 3 ATP. FADH 2. Total = 12 ATP. NADH + H +. GTP.

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ENERGY YIELD

4. Oxaloacetate. 6. Citrate. 4. Malate. Isocitrate. 6. CH 3 C. S-CoA. ~. O. CO 2. CO 2. 4. Fumarate. 5. a -ketoglutarate. 4. 4. Succinate. Succinyl-CoA. ENERGY YIELD. NADH + H +. 3 ATP. 3 ATP. 1 ATP. 2 ATP. NADH + H +. 3 ATP. FADH 2. Total = 12 ATP. NADH + H +. GTP.

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OVERVIEW

Typical Results. Table 1. Affinity-tagged proteins. Gene #. Name. Description. Unique peptides. % sequence coverage. # trials. RPA0176. atpD. ATP synthase beta subunit. 35. 79. 7. RPA0190. sucD. succinyl-CoA synthetase alpha-subunit . 18. 60. 1. RPA0191. sucC.

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