'Forbidden pairs' presentation slideshows

Proteomics via Mass Spectrometry (a bioinformatics perspective)

Proteomics via Mass Spectrometry (a bioinformatics perspective). Vineet Bafna www.cse.ucsd.edu/~vbafna. Nobel Citation 2002. Nobel Citation, 2002. Proteomics via MS. Enzymatic Digestion (Trypsin) +. Fractionation. Q: Sufficient to identify peptides?. Peptide MS.

1.7k views • 148 slides

Proteomics via Mass Spectrometry (a bioinformatics perspective)

Proteomics via Mass Spectrometry (a bioinformatics perspective). Vineet Bafna www.cse.ucsd.edu/~vbafna. Nobel Citation 2002. Nobel Citation, 2002. Proteomics via MS. Enzymatic Digestion (Trypsin) +. Fractionation. Q: Sufficient to identify peptides?. Peptide MS.

1.53k views • 148 slides

Week 3: Functional Genomics via Mass Spectrometry

1.15k views • 115 slides

Week 4: Functional Genomics via Mass Spectrometry

1.15k views • 115 slides

CSE182-L13

CSE182-L13. Mass Spectrometry Quantitation and other applications. 332. 100. 300. 0. 400. 200. 87. The forbidden pairs method. Sort the PRMs according to increasing mass values. For each node u, f(u) represents the forbidden pair Let m(u) denote the mass value of the PRM.

586 views • 51 slides

CSE182-L12

CSE182-L12. Mass Spectrometry Peptide identification. Mass-Charge ratio. The X-axis is not mass, but (M+Z)/Z Z=1 implies that peak is at M+1 Z=2 implies that peak is at (M+2)/2 M=1000, Z=2, peak position is at 501 Quiz: Suppose you see a peak at 501. Is the mass 500, or is it 1000?.

678 views • 43 slides

CSE182-L12

592 views • 43 slides

CSE182-L12

536 views • 40 slides

CSE182-L12

474 views • 40 slides

CSE182-L12

418 views • 34 slides

CSE182-L8

CSE182-L8. Mass Spectrometry. Bio. quiz. What is a gene? What is a transcript? What is translation? What are microarrays? What is a b-ion? What is a y-ion?. 100. 200. 300. 400. 500. De Novo Interpretation: Example. 0 88 145 274 402 b-ions.

315 views • 31 slides

Protein sequencing and Mass Spectrometry

Protein sequencing and Mass Spectrometry. Enzymatic Digestion (Trypsin) +. Fractionation. Sample Preparation. Single Stage MS. Mass Spectrometry. LC-MS: 1 MS spectrum / second. Tandem MS. Secondary Fragmentation. Ionized parent peptide. The peptide backbone.

455 views • 26 slides