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Polymerase Chain Reactions: Denaturation, annealing & elongation

Polymerase Chain Reactions: Denaturation, annealing & elongation. 95 o C 50-65 o C 72 o C Polymerase is thermophilic can withstand high temperature. Exponential growth. Generally take through 20-30 cycles 2(doubling with every cycle) 20-30(cycles) 2 20 = 10 6 copies 2 30 =10 9 copies.

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Fluoro labels - PowerPoint PPT Presentation

Polymerase Chain Reactions: Denaturation, annealing &amp; elongation

Polymerase Chain Reactions: Denaturation, annealing & elongation