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RT-PCR Obtain the internal region of cDNA

RT-PCR Obtain the internal region of cDNA 1. Mix crude RNA or poly(A) RNA, cDNA primer (one of the amplification primers in 7.) 2. Heat at 65 0 C for 3-15 min. 3. Cool on ice 4. Add RT and RT buffer, 42 0 C (37 0 C to 55 0 C, depending on the composition of primer and RNA) for 1 hr.

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