'Dimensional gel electrophoresis' presentation slideshows

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Chapter 6C

Chapter 6C. Proteomics. 25 and 27 February, 2004. Structural and Functional Characterization in the Post-genomic era. Overview. Proteomics examines the collection of proteins present in a given type of cell at a given time.

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Molecular Tools: Molecular Separations *Gel Electrophoresis

Molecular Tools: Molecular Separations *Gel Electrophoresis. Molecular Tools: Molecular Separations *Gel Electrophoresis *One- and Two-Dimensional Gel Electrophoresis of Proteins. Molecular Tools: Molecular Separations *Gel Filtration Chromatography. Molecular Tools:

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Protein Electrophoresis

Protein Electrophoresis. BIT 230. Electrophoresis. Separate proteins based on Size (Molecular Weight - MW) SDS PAGE Isoelectric Point Isoelectric focusing Allows us to characterize (degradation, MW) quantify determine purity of sample compare proteins from different sources

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Identification of regulatory proteins from human cells using 2D-GE and LC-MS/MS

Identification of regulatory proteins from human cells using 2D-GE and LC-MS/MS. Victor Paromov Christian Muenyi William L. Stone. Proteomics techniques used to identify proteins. Obtain cell lysates Run two-dimensional gel electrophoresis (2D-GE) for each sample (in triplicates)

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2D Electrophoresis

Creative Proteomics offers an integrated solution to the identification of low abundance proteins in complex biological sample through 2D electrophoresis. This technique sorts proteins according to two independent properties in two discrete steps. Each spot on the resulting two-dimensional array corresponds to a single protein species in the sample. Thus thousands of different proteins can be separated, and the information is obtained including the protein pI, the apparent molecular weight, and the amount of each protein.

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Supplementary Figure 4 Hori et al.

One-dimensional gel electrophoresis (SDS-PAGE)

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