'Dicer' presentation slideshows

microRNAs

microRNAs. Small molecules, big functions Ali Bazargan. Brief history. The first described microRNA, lin-4 was cloned and characterised as a translational repressor of developmental timing from Caenorhabditis. elegans by Lee et al (1993) and Wightman et al (1993).

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Both prokaryotes and eukaryotes

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Reverse Genetics: interference RNA

Reverse Genetics: interference RNA. Mitchell Bush Mimosa Chau Tayler Fluharty. Summary. Use RNAi to circumvent gene replacement difficulties in Cryptococcus neoformans . Focus on two genes of known phenotype:. CAP59 Codes for product required for polysaccharide capsule.

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What is already known?

RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production. What is already known?. Without rdp1, heterochromatin is lost

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Distinct Roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA Silencing Pathways

Distinct Roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA Silencing Pathways. Lee, S.Y., Nakahara, K., Phan, J.W., Kim, K., He, Z., Sontheimer, E.J., Carthew, R.W. (2004). Cell 117 : 69-81. What is RNAi?.

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Translational Inhibition in Arabidopsis thaliana

Translational Inhibition in Arabidopsis thaliana. Russell Burke Dr. James Carrington Botany and Plant Pathology. Significance. microRNAs are 21 to 24 nucleotide RNAs that serve in post-transcriptional regulation in nearly all eukaryotic organisms

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miRNA Reading: ambion/techlib/hottopics/rnai/ Lecture notes

miRNA Reading: http://www.ambion.com/techlib/hottopics/rnai/ Lecture notes. Components of the RNAi machinery are used for other processes. The first miRNA was discovered in C. elegans . lin-14(lf) mutations caused precocious development.

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Mechanisms of gene silencing by double-stranded RNA

Mechanisms of gene silencing by double-stranded RNA. GENE SILENCING . Transcriptional level. Post-Transcriptional level. Structure of miRNA & siRNA. Pre- miRNA. P. OH. OH. P. siRNA. miRNA after processing . 22 ~ nt bases 5prime P & 3p 2 nt overhang

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MicroRNA pathways Modulate Polyglutamine-induced Neurodegeneration

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