'Cycle sequencing' presentation slideshows

Why bother with a new technology?

Why bother with a new technology?. Current (2005) $1.00 per kilobase 20 bp / second 100 Gb / year. NHGRI Goals $ ? 100x, 5yrs $ ? 10,000x, 15yrs. Collins, Morgan, Patrinos (2003). Growth of GenBank. 60. 50. 40. 30. Base pairs of DNA (billions). Sequences (millions). 20. 10. 0.

431 views • 36 slides

Chapter 10

Chapter 10. DNA Sequencing. Objectives. Compare and contrast the chemical (Maxam/Gilbert) and chain termination (Sanger) sequencing methods. List the components and molecular reactions that occur in chain termination sequencing.

250 views • 24 slides

SEQUENCING-related topics

457 views • 23 slides

PCR DNA sequencing Microarrays Mass-spec

The new biology lab. Laboratory Tools and Techniques. The methods used by molecular biologists to study DNA have been developed through adaptation of the chemical reactions and biological processes that occur naturally in cells

238 views • 23 slides

Cycle Sequencing

Cycle Sequencing. Broad and Long Term Objective. To characterize a single clone from an Emiliania huxleyi cDNA library using sequence analysis. Research Plan. Preparation of Competent Cells and Bacterial Transformation. Growth of Transformant and Plasmid MiniPrep. Cycle Sequencing.

563 views • 13 slides

DNA

DNA. Yong- Gu Lee. Chemical Structure of a Nucleotide. The three parts of the nucleotide building block of DNA are the sugar, the base and the phosphate. The complex of the sugar with the base is called a nucleoside. The distance between base pairs in DNA is 0.34 nm.

191 views • 7 slides