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Confocal scanning laser microscope - PowerPoint PPT Presentation

Microscopy and Cell Structure

Microscopy and Cell Structure

Microscopy and Cell Structure. Chapter 3. Microscope Techniques Microscopes. Microscopes Most important tool for studying microorganisms Use viable light to observe objects Magnify images approximately 1,000x Electron microscope, introduced in 1931, can magnify images in excess of 100,000x

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1.31k views • 103 slides
Microscopy and Specimen Preparation

Microscopy and Specimen Preparation

Microscopy and Specimen Preparation. BIO3124 Lecture #2 . Objectives and reading. Reading: Ch.2 1 . Principles of optical resolution of microorganisms Optical behaviour of light Detection, magnification and resolution How to increase resolution Optical parameters Increasing contrast

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1.15k views • 52 slides
Review of the Microscope

Review of the Microscope

Review of the Microscope. Ch. 1 Biology. Microscopy. Things are not all the same size, are they? Figure shows the resolution of the naked eye, optical (light) microscopy and electron microscopy. History of Microscopy.

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728 views • 47 slides
Microscopes

Microscopes

Microscopes. Compound Bright-Field Light Microscope Maximum Useful Magnification = 1200X Why?. Resolution. Useful magnification is limited by Resolution Resolution is influenced by Numerical Aperture (NA) NA determined by Wavelength of Light and Refractive Index Improve Magnification

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282 views • 13 slides
Microscopes

Microscopes

Microscopes. Compound Bright-Field Light Microscope Maximum Useful Magnification = 1200X Why?. Resolution. Useful magnification is limited by Resolution Resolution is influenced by Numerical Aperture (NA) NA determined by Wavelength of Light and Refractive Index Improve Magnification

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871 views • 12 slides
Presenter: Giri Narasimhan, Ph.D. E.mail: Giri.Narasimhan@memphis

Presenter: Giri Narasimhan, Ph.D. E.mail: Giri.Narasimhan@memphis

Voids. Channels. Mounds. Original CLSM Image. Threshold image. Delete small islands. FD computation (Dilation). Detect edges. Fill out small lakes. Plot Perimeter vs Length. 455 X 480 Pixels. Presenter: Giri Narasimhan, Ph.D. E.mail: Giri.Narasimhan@memphis.edu.

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232 views • 1 slides

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