100+ Cell hybrids PowerPoint (PPT) Presentations, Cell hybrids PPTs

Cell hybrids - PowerPoint PPT Presentation

Categories of cell mutant selections Example Auxotrophs purine-

Categories of cell mutant selections Example Auxotrophs purine- Drug resistance Dominant ouabain R , alpha-amanitin R Recessive 6TG r , BrdU r Antibodies vs. surface components MHC- Visual inspection G6PD-, Ig IP- FACS = fluorescence?activated cell sorter DHFR-

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464 views • 46 slides

Extinction

Extinction. Loss of tissue-specific gene expression in somatic cell hybrids. 1966- Loss of cell pigment in somatic hybrids- Davidson and Yamamoto 1970s- Albumin extinction- time course/reversability- Petit and Weiss 1984- Identification of extinguisher locus TSE-1- Killary and Fournier

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473 views • 32 slides

r-RNA silencing

r-RNA silencing. A. Introduction- rRNA produced in nucleoli Recall function of rRNA- interact with proteins to produce ribosomes for mRNA translation into proteins. m. Nucleolar dominance- in interspecific cell hybrids, only rRNA genes inherited from one parent are transcribed.

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437 views • 8 slides

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Cell hybrids - PowerPoint PPT Presentation

Categories of cell mutant selections Example Auxotrophs purine-

Extinction

r-RNA silencing