100+ Binding sequence PowerPoint (PPT) Presentations, Binding sequence PPTs

Binding sequence - PowerPoint PPT Presentation

RT-PCR Obtain the internal region of cDNA

RT-PCR Obtain the internal region of cDNA 1. Mix crude RNA or poly(A) RNA, cDNA primer (one of the amplification primers in 7.) 2. Heat at 65 0 C for 3-15 min. 3. Cool on ice 4. Add RT and RT buffer, 42 0 C (37 0 C to 55 0 C, depending on the composition of primer and RNA) for 1 hr.

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756 views • 54 slides

Essentials of Glycobiology April 14, 2002 Jeff Esko

Essentials of Glycobiology April 14, 2002 Jeff Esko. Glycosaminoglycan-Binding Proteins. Glycosyltransferases and modifying enzymes Plant Lectins: Con A, PHA, WGA, Ricin, and many others Animal Lectins: P,C.S, and I-type, chaperone proteins Glycosaminoglycan-binding proteins

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432 views • 33 slides

How do we represent the position specific preference ?

G: 5 -> 71% S: 1 -> 14 % C: 1 -> 14 %. Basic concept of motif identification 2. How do we represent the position specific preference ?. BID_MOUSE I A R H L A Q I G D E M BAD_MOUSE Y G R E L R R M S D E F BAK_MOUSE V G R Q L A L I G D D I

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327 views • 19 slides

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Binding sequence - PowerPoint PPT Presentation

RT-PCR Obtain the internal region of cDNA

Essentials of Glycobiology April 14, 2002 Jeff Esko

How do we represent the position specific preference ?