'2d dige silver stain' presentation slideshows

host cell protein analysis mass spectrometry

Host Cell Proteins (HCPs) are a heterogeneous, complex group of proteins that derived from the host cell. HCPs differ significantly in molecular mass, isoelectric point, hydrophobic properties, and structures. Figure 1 shows a harvested product pool of bacterial and mammalian fermentation by two-dimensional difference gel electrophoresis (2D-DIGE). Identification and quantification HCPs is very important in the development of biopharmaceutical products to ensure product purity and patient safety. Protease of HCPs can influence the protein pattern in culture and influence the purification process. In addition, HCPs can undergo post-translational modifications that make quantification and characterization more difficult. Moreover, high HCP variance results in different biochemical properties which could cause an immune response or allergic reactions even anaphylactic shock.

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