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GENE TRANSFER AND GENETIC ENGINEERING

GENE TRANSFER AND GENETIC ENGINEERING. CHAPTER 8. Gene Transfer. Vertical to offspring Lateral (horizontal) to others in same generation. Bacterial Gene Transfer and Recombinant DNA Technology. Transformation Griffith’s experiment. Transformation. Mechanism Significance.

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GENE TRANSFER AND GENETIC ENGINEERING

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  1. GENE TRANSFER AND GENETIC ENGINEERING CHAPTER 8

  2. Gene Transfer • Vertical to offspring • Lateral (horizontal) to others in same generation

  3. Bacterial Gene Transfer and Recombinant DNA Technology • Transformation • Griffith’s experiment

  4. Transformation • Mechanism • Significance Recombination?

  5. Transduction • Mechanism • Significance Transducing phages: p1, p22 Can be used in genetic mapping http://jb.asm.org/cgi/reprint/174/23/7876

  6. Conjugation • Mechanism • Significance Conjugation pilus Also used for mapping

  7. Plasmids • F plasmid (conjugation) • Resistance plasmids • Virulence plasmids

  8. Plasmids (cont.) • Tumor-inducing plasmids • Agrobacterium tumefaciens and plants • Plasmids conferring novel catabolism Degradation pathways on a plasmid

  9. Genetic Engineering • Genetic fusions • reporter fusions • promoter fusions

  10. Genetic Engineering • Protoplast fusion • electroporation

  11. Genetic Engineering (cont.) • Gene Amplification • Recombinant DNA cloning

  12. Applications • Medical • Industrial • Agricultural • Hybridomas

  13. Risks and Benefits Frankenstein? Frankenfoods?

  14. Recombinant DNA technology • Plasmids- autonomous replicating extrachromasomal DNA • restriction enzymes

  15. Viral technology- phage cloning

  16. Homologous recombination Used to make gene knockouts in bacteria and yeast

  17. Transposons - jumping genes

  18. Evidence for extensive transfer of antibiotic resistance genes inBacteroides sp. http://mcb.illinois.edu/faculty/profile/abigails

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