Agarose Gel Visualization of Restriction Enzyme Digest. Bridges 2014. Gel Electrophoresis: Main Principles. Separate DNA fragments by size Smaller DNA fragments move faster Run a “size marker” to compare size of separated fragments DNA moves through gel due to electric field
100bp size marker
DNA needs electric field to move
Power source supplies this electric field
Set power source to the voltage you want and let run for specified amount of time
Make sure positive goes to positive and negative to negative
Make sure wells are at negative end!