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PCR - Polymerase Chain Reaction. Allows for amplification of a DNA sequence by repeated cycles of replication. very similar to DeptNA replication in cell, except: DNA strands separated by heat synthetic DNA primers used instead of RNA primers Taq Polymerase instead of DNAP I & III

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Pcr polymerase chain reaction

PCR - Polymerase Chain Reaction

Allows for amplification of a DNA sequence by repeated cycles of replication




Rflp restriction fragment length polymorphism

RFLP - Restriction Fragment Length Polymorphism millions of copies in a short period of time

using restriction enzymes to digest DNA fragments and compare differences between individuals


Polymorphism
Polymorphism millions of copies in a short period of time

  • difference in DNA sequence between individuals of same species

  • fragments of DNA have microsatellites or VNTR’s (variable number tandem repeats), short sequences (2-6 bp) that repeat over and over



Southern blotting
Southern Blotting except identical twins

  • transfers DNA from gel onto nylon membrane while keeping DNA fragments in place

  • probe the membrane with radioactive labelled complimentary sequence

  • probe will attach to DNA at certain locations (hybridization)



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