Pcr polymerase chain reaction
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PCR - Polymerase Chain Reaction. Allows for amplification of a DNA sequence by repeated cycles of replication. very similar to DeptNA replication in cell, except: DNA strands separated by heat synthetic DNA primers used instead of RNA primers Taq Polymerase instead of DNAP I & III

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PCR - Polymerase Chain Reaction

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Pcr polymerase chain reaction

PCR - Polymerase Chain Reaction

Allows for amplification of a DNA sequence by repeated cycles of replication


Pcr polymerase chain reaction

  • very similar to DeptNA replication in cell, except:

  • DNA strands separated by heat

  • synthetic DNA primers used instead of RNA primers

  • Taq Polymerase instead of DNAP I & III

  • 2 parent strands synthesised continuously


Pcr polymerase chain reaction

  • PCR allows a single sequence of DNA to be amplified to millions of copies in a short period of time

  • used in forensic criminal investigations, medical diagnostics & genetic research

  • Cutting, Pasting, & Copying DNA & the Recombinant DNA Controversy


Rflp restriction fragment length polymorphism

RFLP - Restriction Fragment Length Polymorphism

using restriction enzymes to digest DNA fragments and compare differences between individuals


Polymorphism

Polymorphism

  • difference in DNA sequence between individuals of same species

  • fragments of DNA have microsatellites or VNTR’s (variable number tandem repeats), short sequences (2-6 bp) that repeat over and over


Pcr polymerase chain reaction

  • length and number of VNTR’s are different in every person except identical twins

  • known as ‘DNA fingerprinting’

  • restriction enzymes will give different length DNA fragment for each person


Southern blotting

Southern Blotting

  • transfers DNA from gel onto nylon membrane while keeping DNA fragments in place

  • probe the membrane with radioactive labelled complimentary sequence

  • probe will attach to DNA at certain locations (hybridization)


Pcr polymerase chain reaction

  • when exposed to x-ray film, places where probe attached to DNA shows dark bands (audiogram)

  • pattern on audiogram can be used to compare suspect’s DNA to DNA found at crime scene

  • Use of DNA Analysis in Identification, Health, & Human Origins


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