Principle of fluorescence. Outline. Luminescence : fluorescence or phosphorescence? Jablonski diagram Characteristics of fluorescence emission Fluorescence lifetime and quantum yield Quantum mechanic behind Quenching Beer-Lambert law Biochemical fluorescence. phosphorescence.
Principle of fluorescence
Pic. from : http://www.glassner.com/andrew/cg/research/fluphos/fluphos.htm
Pic. From : http://en.wikipedia.org/wiki/Image:Fluorescent_minerals_hg.jpg
KD is stern-volmer quenching constant, kq is bimolecular constant, τ0 is unquenched lifetime.
density indicates us the
absorption of fluorescent
where c is concentration (M) and ε is the extinction coefficient (M-1cm-1)
4.Lissamine rhodamine B
Red: rhodamine dextran blue: hoechst33342
of GFP is used in cell
by injection or fusion