SEMINAR-III 28-09-09 SPEAKER KALPANA. Impedimetric monitoring of cell attachment on interdigitated microelectrodes Bin-Wha Chang a , b , Che-Hsiung Chena, Shin-Jyh Ding c , David Chan-Hen Chend, Hsien-Chang Changa , ∗
Impedimetric monitoring of cell attachment on interdigitated microelectrodes
Bin-Wha Changa,b, Che-Hsiung Chena, Shin-Jyh Ding c, David Chan-Hen Chend, Hsien-Chang Changa,∗
aInstitute of Biomedical Engineering, National Cheng-Kung University, Tainan 701, Taiwan, ROC
bDepartment of Healthcare Administration, Hung-Kuang University, Taichung 433, Taiwan, ROC
cInstitute of Dental Materials, Chung-Shan Medical University, Taichung 402, Taiwan, ROC
dInstitute of Veterinary Microbiology, National Chung-Hsing University, Taichung 402, Taiwan, ROC
Schematic diagram of the impedimetric system:
2.1. Impedimetric measuring chamber with microelectrodes microelectrodes
on a glass slide (26mm × 76mm; Fig. 1b) by the wet-etching process.
(b) schematic diagram of the interdigitated microelectrode plate; (c) a photograph of MDCK cells grown on the microelectrode plate.
2.2. Culturing cells in the measuring chamber
2.3. Impedance analyzing system
3. Results and discussion by dipped-coating procedure.
Monitoring of admittance during cell attachment:
` Determination of cell density using low-frequency conductance signal:
(Fig. 4) with different cell densities using chambers with bare microelectrodes:
The following equation was established using chambers with electrodes coated with RGD-C peptide (n = 20; Fig. 4):
4. Conclusions experiments
quantified by the proposed system.