Evolution at the nucleotide level
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Evolution at the Nucleotide Level. Rapid Evolution of HIV/SIV and Microsatellites in Candida albicans. Matthew D. Pagel NSCORT/Exobiology Fellow Christopher Wills Lab Department of Biology, University of California - San Diego. HIV/SIV Phylogenetic Trees. A Model for Rapid Evolution.

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Evolution at the Nucleotide Level

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Evolution at the Nucleotide Level

Rapid Evolution of HIV/SIV and Microsatellites in Candida albicans

Matthew D. Pagel

NSCORT/Exobiology Fellow

Christopher Wills Lab

Department of Biology, University of California - San Diego


HIV/SIV Phylogenetic Trees

A Model for Rapid Evolution


Sample Sequence Alignment


HIV/SIV Env gene Neighbor-Joining Tree(from Yellow Fever, Black Goddessby C. Wills)


Neighbor-Joining Tree of Env gene of HIV/SIV - NCBI/Los Alamos Complete Sequences


Pol Neighbor-Joining Tree

HIV1

HIV2


Neighbor-Joining Tree of Gag Gene of HIV/SIV


NJ Tree of Gag after Unverified Sequences Removed


Cloning of Candida albicans genes involved in pathogenicity

Plasmid Construction of Erk1


Microsatellite Slippage Mutations (Glutamine)


The Genetic Code--The Translation of mRNA to Amino Acids--


Recombination-Mediated Plasmid Construction


Action of Restriction Enzymes(Eco RI example clipped from Molecular Cell Biology CD-Rom)


Plasmid Constructionvia Restriction Digest


Blue/White Screen


Future Steps

  • Site-directed mutagenesis producing variant microsatellite length

  • Re-insertion of plasmid into knockout strains

  • Analysis of morphological form (hyphal)


Acknowledgements

Chris Wills

Nikki Freed

Dave Metzgar

Sol Guiterrez

Hattie Oswald

Lois Lane

Bill Baity

Carlos Flores, Vincent Starai, Carlos Peralta, & William Engels

The NSCORT/Exobiology Program


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