Evolution at the nucleotide level
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Evolution at the Nucleotide Level. Rapid Evolution of HIV/SIV and Microsatellites in Candida albicans. Matthew D. Pagel NSCORT/Exobiology Fellow Christopher Wills Lab Department of Biology, University of California - San Diego. HIV/SIV Phylogenetic Trees. A Model for Rapid Evolution.

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Evolution at the Nucleotide Level

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Evolution at the nucleotide level

Evolution at the Nucleotide Level

Rapid Evolution of HIV/SIV and Microsatellites in Candida albicans

Matthew D. Pagel

NSCORT/Exobiology Fellow

Christopher Wills Lab

Department of Biology, University of California - San Diego


Hiv siv phylogenetic trees

HIV/SIV Phylogenetic Trees

A Model for Rapid Evolution


Sample sequence alignment

Sample Sequence Alignment


Hiv siv env gene neighbor joining tree from yellow fever black goddess by c wills

HIV/SIV Env gene Neighbor-Joining Tree(from Yellow Fever, Black Goddessby C. Wills)


Evolution at the nucleotide level

Neighbor-Joining Tree of Env gene of HIV/SIV - NCBI/Los Alamos Complete Sequences


Pol neighbor joining tree

Pol Neighbor-Joining Tree

HIV1

HIV2


Evolution at the nucleotide level

Neighbor-Joining Tree of Gag Gene of HIV/SIV


Evolution at the nucleotide level

NJ Tree of Gag after Unverified Sequences Removed


Cloning of candida albicans genes involved in pathogenicity

Cloning of Candida albicans genes involved in pathogenicity

Plasmid Construction of Erk1


Microsatellite slippage mutations glutamine

Microsatellite Slippage Mutations (Glutamine)


The genetic code the translation of mrna to amino acids

The Genetic Code--The Translation of mRNA to Amino Acids--


Recombination mediated plasmid construction

Recombination-Mediated Plasmid Construction


Action of restriction enzymes eco ri example clipped from molecular cell biology cd rom

Action of Restriction Enzymes(Eco RI example clipped from Molecular Cell Biology CD-Rom)


Plasmid construction via restriction digest

Plasmid Constructionvia Restriction Digest


Blue white screen

Blue/White Screen


Future steps

Future Steps

  • Site-directed mutagenesis producing variant microsatellite length

  • Re-insertion of plasmid into knockout strains

  • Analysis of morphological form (hyphal)


Acknowledgements

Acknowledgements

Chris Wills

Nikki Freed

Dave Metzgar

Sol Guiterrez

Hattie Oswald

Lois Lane

Bill Baity

Carlos Flores, Vincent Starai, Carlos Peralta, & William Engels

The NSCORT/Exobiology Program


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