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Evolution at the Nucleotide Level. Rapid Evolution of HIV/SIV and Microsatellites in Candida albicans. Matthew D. Pagel NSCORT/Exobiology Fellow Christopher Wills Lab Department of Biology, University of California - San Diego. HIV/SIV Phylogenetic Trees. A Model for Rapid Evolution.

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Evolution at the nucleotide level

Evolution at the Nucleotide Level

Rapid Evolution of HIV/SIV and Microsatellites in Candida albicans

Matthew D. Pagel

NSCORT/Exobiology Fellow

Christopher Wills Lab

Department of Biology, University of California - San Diego


Hiv siv phylogenetic trees
HIV/SIV Phylogenetic Trees

A Model for Rapid Evolution



Hiv siv env gene neighbor joining tree from yellow fever black goddess by c wills
HIV/SIV Env gene Neighbor-Joining Tree(from Yellow Fever, Black Goddessby C. Wills)



Pol neighbor joining tree
Pol Neighbor-Joining Tree Alamos Complete Sequences

HIV1

HIV2




Cloning of candida albicans genes involved in pathogenicity

Cloning of Alamos Complete SequencesCandida albicans genes involved in pathogenicity

Plasmid Construction of Erk1


Microsatellite slippage mutations glutamine
Microsatellite Slippage Mutations ( Alamos Complete SequencesGlutamine)


The genetic code the translation of mrna to amino acids
The Genetic Code Alamos Complete Sequences--The Translation of mRNA to Amino Acids--



Action of restriction enzymes eco ri example clipped from molecular cell biology cd rom
Action of Restriction Enzymes Alamos Complete Sequences(Eco RI example clipped from Molecular Cell Biology CD-Rom)


Plasmid construction via restriction digest
Plasmid Construction Alamos Complete Sequencesvia Restriction Digest


Blue white screen
Blue/White Screen Alamos Complete Sequences


Future steps
Future Steps Alamos Complete Sequences

  • Site-directed mutagenesis producing variant microsatellite length

  • Re-insertion of plasmid into knockout strains

  • Analysis of morphological form (hyphal)


Acknowledgements
Acknowledgements Alamos Complete Sequences

Chris Wills

Nikki Freed

Dave Metzgar

Sol Guiterrez

Hattie Oswald

Lois Lane

Bill Baity

Carlos Flores, Vincent Starai, Carlos Peralta, & William Engels

The NSCORT/Exobiology Program


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