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CENTRAL STERILIZATION AND SUPPLIES DIVISION

CENTRAL STERILIZATION AND SUPPLIES DIVISION. Infection Control Nursing Officer Infection Control Unit Teaching Hospital Jaffna. Definition of terms. Sterilization – complete elimination or destruction of all forms of microbial life (including spores)

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CENTRAL STERILIZATION AND SUPPLIES DIVISION

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  1. CENTRAL STERILIZATION AND SUPPLIES DIVISION Infection Control Nursing Officer Infection Control Unit Teaching Hospital Jaffna.

  2. Definition of terms • Sterilization – complete elimination or destruction of all forms of microbial life (including spores) • Disinfection – elimination of micro-organisms on inanimate objects with the exception of bacterial spores • Antisepsis – prevention of infection of tissues and body surfaces by application of germicides • Cleaning – the removal of visible soil (eg; inorganic and organic material) from objects and surfaces

  3. Disinfection and sterilization practices are essential for ensuring that medical and surgical instruments do not transmit infectious pathogens to patients

  4. Instruments and items for patient care are divided into 3 categories based on the degree of risk of infection involved in the use of the items • 1. Critical/ high risk items • 2. Semicritical/ intermediate risk items • 3. Non critical/ low risk items

  5. Critical / high risk items • Objects that enter sterile body tissue or vascular system • Any microbial contamination could result in disease transmission • Must be sterile

  6. Semi-critical / intermediate risk items • Items that come in contact with intact mucous membranes • Generally resistant to infection by common bacterial spores but susceptible to other micro-organisms • Need not be sterile; disinfectionacceptable

  7. Non-critical / low risk items • Items that come into contact with intact skin but not mucous membranes • Intact skin is an effective barrier to most micro-organisms, hence low risk of disease transmission • Thorough cleaning is adequate with low level disinfection as necessary

  8. Factors influence the sterilization process • The temperature of sterilization • The period of sterilization • Inactivation by organic matter • Whether the article was cleaned before sterilization •  The sterilization cycle can be divided into three periods: • a.Heating up period • b. Holding period • c. Cooling period

  9. CENTRAL STERILE SUPPLY DIVISION

  10. Objective • To reduce hospital associated infections and protect patients, employees, health care students and visitors.

  11. The functional flow of activities in CSSD • RINSING • CLEANING • DRYING • INSPECTION AND ASSEMBLY • PACKAGING • LABELLING • STERILIZATION • STORAGE • DISTRIBUTION

  12. RINSING • Rinsing of articles after use should not be permitted in patient care areas unless carried out by a trained member of the staff.

  13. CLEANING • All reusable medical devices should be thoroughly cleaned prior to disinfection or sterilization.

  14. DRYING • All articles should be dried appropriately

  15. INSPECTION AND ASSEMBLY • Each item should be inspected for functionality, defects, breakage and then appropriately assembled.

  16. PACKAGING • Articles should preferably be packed in porous material.

  17. LABELLING • Each pack should be marked with nomenclature of the article, contents of the pack, initials of the person who packed it, date and initials of the person who carried out the sterilization.

  18. STORAGE • Should be properly managed separately for sterile and non-sterile stores. For sterile goods, clean room conditions should be followed. • Sterile pack should be stored 20-25 cm from the floor, 45-50 cm from the ceiling, 15-20 cm on out side of the wall.

  19. DISTRIBUTION • Refers to clean and dirty articles exchange system. A program should be established for the collection of used items from patient care areas and distribution of sterilized goods.

  20. Moist heat sterilization • Moist heat kills microorganisms by coagulating and denaturing enzymes and structural proteins • Applied in the form of steam • Steam under pressure is used to achieve a higher temperature • 4 parameters of steam sterilization • Steam – saturated and dry steam without air • Pressure – 2.4 bar / 15lb • Temperature – 1210C • Time - 20 min Autoclaving at 1210C at 15 lb pressure for 20 min is acceptable for routine hospital use

  21. Moist heat sterilization… Advantages • Non toxic • Inexpensive • Rapid action • Good penetration ability

  22. Sterilization by dry heat • Dry heat penetrates less well and is less effective than moist heat • Consequently, higher temperatures and longer periods are required for sterilization with dry heat • Used for sterilization of -sharp cutting instruments and -laboratory glass wear -powder, oil

  23. Sterilization by dry heat • Total cycle may be several hours, hence hot air ovens should have • Time lock on the door - items cannot be added or removed during, the cycle • Fan to distribute the heat evenly Dry heating at 1600C for for 2 hours is acceptable for routine hospital use

  24. Monitoring of sterilization • Mechanical indicators –Observe the machine's dials and gauges - temperature, pressure, time • Chemical indicators – changes colour on exposure to the appropriate sterilization cycle Autoclave strips Bowie Dick test

  25. Monitoring of sterilization… • Biological indicators – bacterial spores which require high temperatures to lose viability – spore strips, self contained vials • Geobacillus stearothermophilus – for steam or chemical vapor sterilization • Bacillus subtilis -for dry-heat or ethylene oxide gas sterilization

  26. Storage • Once sterilized, the instruments should be maintained in a sterile state until they are used again • Improper storage would result in a break of the 'chain of Sterility' • The expected 'shelf-life' of a sterile package is depend on the type of area in which it is stored and the material used for packaging • A closed, protected area with a minimal airflow such as a cabinet or drawer that can easily be disinfected is preferable to an open stacking system

  27. Disinfection Methods • Heat – • Pasteurization - 63-66 0C for 30 min 72 0C for 15 sec. (the flash method) • Boiling - bacterial spores can survive • Physical – ultrasonic, UV radiation - used for removing debris (cleaning) prior to autoclaving • Chemical • Disinfectant used for one purpose may not be equally effective for another • The antimicrobial activity falls in the presence of organic debris

  28. Disinfection & Sterilization • 2% Glutaraldehyde(cidex) - 2% Alkaline solution - Needs activation - Sterilization time – 10 hours - Disinfection time – 20 mins - Activated solution – 2 weeks/20 times - Sterilization/HLD of heat sensitive items - Irritant to skin, eyes & respiratory tract - Should be handled in a well ventilated area - Should wear protective clothing - Fixation of protein

  29. Disinfection & Sterilization (cont.) • Para Safe - Combination of per oxygen compound, activator & stabilizer - Non-corrosive - Non-irritant to skin - Eye irritation - No toxic residues - Expensive - Stable for 24 hours only

  30. Disinfection & Sterilization (cont.) • Chlorine releasing agents - Sodium hypochlorite, Calcium hypochlorite, Chlorine granules - Active against bacteria, viruses, fungi & some spores - Less active against mycobacteria - Contact time 20-30 mins - Rapidly inactivated by organic matter - Corrosive to metal - Do not add powder to water - Avoid contact with acid/acidic body fluids e.g.- urine - Avoid contact with skin, eyes & mucus membrane - Unstable - daily preparation is necessary - Blood & body fluid spillages – 1% solution (10000 ppm) - General environmental cleaning – 0.1% (1000 ppm)

  31. Disinfection & Sterilization (cont.) • Alcohol - 70% - 90% Ethyl alcohol/ Isopropyl alcohol - Rapidly active against vegetative bacteria & mycobacteria - Viruses – variable activity - Not sporicidal - Only on clean surfaces - Disinfection of items that cannot be immersed in other disinfectants e.g.- electric equipment - Skin anti-sepsis

  32. Disinfection & Sterilization (cont.) • Chlorhexidine – 4%-5% (Hibiscrub/Hibitane) - Active against Gram positive organisms & fungi - Poorly active against Gram negative organisms - Non toxic - Good residual activity - Pre operative skin disinfection/Surgical hand washing - Savlon – 1.5% Chlorhexidine + 15% Cetrimide Poor bactericidal activity

  33. Disinfection & Sterilization (cont.) • Chlorhexidine – 4%-5% (Hibiscrub/Hibitane) - Active against Gram positive organisms & fungi - Poorly active against Gram negative organisms - Non toxic - Good residual activity - Pre operative skin disinfection/Surgical hand washing - Savlon – 1.5% Chlorhexidine + 15% Cetrimide Poor bactericidal activity

  34. Principles of disinfection • Objects that cannot be heat-treated, should be disinfected by chemicals • Clean the article before disinfection • Dilution of concentrates should be accurately measured • Consider the range of activity, toxicity and cost • Beware of inactivating substances • Use fresh disinfectant solutions • Use clean containers • Never 'Top up' disinfection containers • Item should kept in contact with the disinfectant for the required time period and no longer than that

  35. Chemical disinfectants… Factors affecting the effectiveness/usefulness of chemical disinfection • Range of activity • Satisfactory contact • Concentration • pH- alkaline for gluta. and acid for phenols • Neutralization- organic matter, hard water, soap • Stability • Speed of action- high-hypochlorite • Cost • Potency- high, intermediate, low • Lack of adverse effects

  36. Commonly used disinfectants Antimicrobial activity

  37. Chemical agents used as high-level disinfectants

  38. Sterilizing medical items High risk

  39. Intermediate risk -High level disinfection

  40. Low risk- cleaning and low level disinfection

  41. Management of a blood spill • Wear heavy duty gloves • Soak the spill with an absorbent material • Cover with 1% Sodium hypochlorite • Leave for 20 minutes • Clean

  42. Take home message In failure of sterilization and disinfection • Increase morbidity and mortality • Longer hospital stay • Expensive antibiotics • Increase work load- doctors, nurses, laboratory • Extra cost to the state! Apply your knowledge appropriately

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