Lentiviral vectors
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LENTIVIRAL VECTORS. introduction. Lenti - latent-slow Retroviridae Enveloped , spherical viruses with a genome size of ~ 8kb Can replicate in the non dividing cells-making them one of the most efficient gene delivery vector

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LENTIVIRAL VECTORS

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Lentiviral vectors

LENTIVIRAL VECTORS


Introduction

introduction

  • Lenti- latent-slow

  • Retroviridae

  • Enveloped , spherical viruses with a genome size of ~ 8kb

  • Can replicate in the non dividing cells-making them one of the most efficient gene delivery vector

  • Although transmission is generally via infectious particles, lentiviruses are capable of infecting neighboring cells in direct contact with the host cells, without having to form extracellular particles.


Members

members

  • Human Immunodeficiency Virus 1 and 2

  • Simian Immunodeficiency Virus

  • Feline Immunodeficiency Virus

  • Bovine immunodeficiency virus

  • Equine infectious anemia virus

  • Caprine arthritis

  • Caprine encephalitis virus

  • Puma lentivirus


How to make a viral vector from the virus

HOW TO MAKE A VIRAL VECTOR FROM THE VIRUS?


Lentivirus genome

LENTiVIRUS GENOME


Process

process


The steps involved

The steps involved


Experimentally

experimentally


No helper virus no goi

NO HELPER VIRUS- NO GOI!


The problem genetic recombination

THE PROBLEM-GENETIC RECOMBINATION!


Solution

SOLUTION


Vaccines currently available

Vaccines currently available


Safety issues

SAFETY ISSUES

  • Lentiviruseshave a high mutation rate due to low fidelity of the lentivirusreverse transcriptase and the absence of proofreading activity. This results in the constant generation of mutants that escape from surveillance either by antibodies or specific T -cells.

  • Following integration of the proviral DNA into the host genome lentiviruses can remain in a quiescent state, invisible for the immune system. The initial site of infection is usually at the mucosae of the genital tracts or the intestine where the virus can replicate in the dendritic cells.

  • To limit viral replication in this initial stage of infection the induction of an effective mucosal immune response will be necessary. Unfortunately most vaccine strategies pursued currently do not induce mucosal immune responses.

  • Another problem in the immunologic control of lentivirus infection is that they destroy or interfere with the functioning of T helper cells which have a key role in the immune system.


Thank you

Thank you


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