Chapter 16 oncogene anti oncogene and growth factor
Sponsored Links
This presentation is the property of its rightful owner.
1 / 69

Chapter 16 Oncogene, Anti-oncogene and Growth Factor PowerPoint PPT Presentation


  • 322 Views
  • Uploaded on
  • Presentation posted in: General

Chapter 16 Oncogene, Anti-oncogene and Growth Factor. 第十 六 章 癌基因、抑癌基因与生长因子. 引言. 据估计,大约有三分之一的人在生命的某一阶段要遭遇癌症。 迄今发现的癌症有 200 多种,我国最为常见和危害性最严重的肿瘤为 肺癌、食管癌、胃癌、大肠癌、肝癌、乳腺癌、鼻咽癌、宫颈癌和淋巴瘤、白血病 。 根据卫生部肿瘤防治办公室提供的 2006 年我国肿瘤发病率和十大恶性肿瘤发病率排序显示, 肺癌 、 乳腺癌 分别位居 男、女性恶性肿瘤发病首位 ,男女恶性肿瘤 死亡率最高 的均为 肺癌 。.

Download Presentation

Chapter 16 Oncogene, Anti-oncogene and Growth Factor

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Chapter 16 Oncogene, Anti-oncogene and Growth Factor


  • 200

  • 2006


  • tumor



(Oncogene)


Peyton Rous and Rous sarcoma virus


1966 G.

  • 1910

  • 30()


  • 1932Shope


  • 1950

  • Rous(acute transforming retrovirusRNA)


Rous

196687


  • Bishop1980

  • Rousv-onc


Virus-like particles assembled in vitro with purified Rous sarcoma virus Gag protein


LTR

gag

pol

env

src

LTR

* RSV, Rous sarcoma virus

  • LTR (long terminal repeat)DNA

  • srcRSV


LTR

LTR

GAG

POL

ENV

LTR

ONC

LTR

GAG

POL

ENV

  • v-onc)


RNA

RNA

RNA-DNA

RNA

Proto-oncogenes

DNA

DNA

RNA-


    • virus oncogene,v-onc

    • (cellular-oncogene, c-onc)proto-oncogene, pro-onc


  • (house-keeping gene)

    • ;


v-oncc-onc

  • v-onc

  • v-oncc-onc

  • v-onc



  • (1) srcavian Rous sarcoma virusRous

    • erbavian erythroblastosis virus

    • sissimian sarcoma virus

    • rasrat sarcomas

  • (2) 3

  • erbA--

  • erbB--


  • src

  • ras p21GTPGTP

  • myc DNA

  • sis p28(

  • erb

  • myb


1GFPDGFEGFTGF-2

2

3

4



1982 3 EJ c-H-ras356

1 2 3 4 5 6 7 8 9 10 11 12 13 14

H-rasATG ACG GAA TAT AAG CTG GTG GTG GTG GGC GCC GGC GGT GTG

H-ras ATG ACG GAA TAT AAG CTG GTG GTG GTG GGC GCC GTC GGT GTG

p21Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Ala Val

p21 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Val Ala Val


  • Ras

GDP

GTP

Pi

Ras

GTP

GDP-GTP

Ras

Ras

GDP

GTP


  • HarreyKirfenH-rasK-rasN-ras12136112


GC GC GC

GCAC AT

CG CG CG

12

O6

p21


  • DNAc-mycc-myc


avian leucosis virusALV

c-myc

exon-3

exon-1

exon-2

exon-3

exon-1

exon-2

LTR

LTR

ALV

DNALTR

c-myc

exon-3

exon-2

LTR

exon-1

ALV

c-myc30-100

exon-3

exon-2

exon-1

LTR

ALV


  • DNA5%


DNA

      • DNA

  • DNA

  • DNA



c-myc

  • c-mycALV

  • c-mycG0G1c-myc

  • c-mycc-myc

  • 1985Shibuyac-myc


    • 1960NowellHungeforaPhiladelphia chromosome, Ph

    • Ph


c-bcr

c-abl

c-ablbcr

c-abl


  • 2008121


Section 2 Anti-oncogene


  • anti-oncogene



  • 23

    • Rbretinoblastoma

    • Bcl-2B-cell lymphoma B

    • p5353KD

    • p

    • ras: Ras, p21



retinoblastoma gene,Rb


retinoblastomaRb

  • 2


1. Rb

  • Rbretinoblastoma gene

  • 1986

  • Rb 13q14200kb2726mRNA4.7kb,105-110kDp105-RB


2. Rb


DNA

Rb

P

DNA

G0 G1

Rb

E2F

cyclin dependence protein kinase, CDK)

S

E2F

mRNA


Rb

E-2F

P

P105-Rb

G0 G1

DNA

Rb

S

E-2F

DNA

mRNA


RB

  • DNARBRB

    • E1ASV40TE7


p53

  • cancer suppressive gene ;

  • Pubmed4;

  • 50%p53;

  • 1989.


P53

DNA

DNA

p53

  • p5320kb17p131139353kd;

  • P53NC


p53

  • p53 DNA


p53

p53p2121kDp21WAF1/CIP1p21CDKG1

DNA

DNA p53G1DNA


DNA

  • p53A(RPA)

  • p53RPARPADNAS

  • p53DNA

  • p53 G1DNA


p21

p53

A

p53

DNA

p53

p53

p21

G1


p53

5-8


<Humphrey)

1967


P53

1994PCR1967p53

p53227


  • MycRas


ER FearonB Vogelstein , 1990


APC

  • APC1986Herrera5q212843

  • Dihlmann500(familial adenomatous polyposisFAP)APC8OAPC

  • APC


K-ras

  • K-ras

  • GTP

  • 5O%K-ras

  • K-ras


p53

  • p5317pl3

  • l7p53p53

  • 17


point

    • Rbp53


  • p53


  • Login