26nm. Samantha Downey, Mikhail Rudenko, Holger Schmidt, David Deamer Jack Baskin School of Engineering, University of California, Santa Cruz Surf-IT, Summer 2008. The Solution:
Samantha Downey, Mikhail Rudenko, Holger Schmidt, David Deamer
Jack Baskin School of Engineering, University of California, Santa Cruz
Surf-IT, Summer 2008
The following is the data received from the various trials listed under Method. One can see that adding BSA at pH 9 reduces adhesion of phage to SiN, but phage alone shows little adhesion at pH 8. Therefore, using a buffer of ~pH 8 to transport the molecule through the channel is ideal.
Q-β Bacteria Phage injecting its RNA into a cell. Q-β Bacteria Phage diameter with negative charges around the capsid.
1. BSA has a high affinity for the silicon at pH 9, which is also true for the phage.
a. Phage bound to the wall of the channel, b. phage bouncing off the surface at optimal pH (see graph 2) , c. phage bouncing off BSA molecule on SiN surface(see graph 3).
Single molecule detection apparatus and close up showing molecules clumping together and also adhering to the walls of the channel.
2. Phage alone at three pH levels showing how adhesion increases as the pH nears 9. Corresponds to image (b.) to the left.
3. Phage with BSA which shows that Phage does not adhere with BSA. This corresponds with image (c.) on the left.