Stem cells (and how to make your own). Mr Dare TSI Friday Feb 11 1.30 in S1. What are stem cells?. What can they do?. Why can’t all our cells do this?. Pluripotency genes are switched off Why? So you get… How? Epigenetics!. Transcription is controlled by chemically changing the DNA.
(and how to make your own)
1.30 in S1
Acetyl groups remove the positive charge from the histones
Dr Shinya Yamanaka
New master switches
But will they act like ES cells – will they have pluripotency?
MEF cells – stem cells for skin
Mouse embryonic stem cell (MES) colony
The product – skin cells transformed into cells which are just like ES cells
So it looks like these cells are pluripotent
SCID mice with teratomas from injected transformed cells
Inner cell mass
Generation of ES cells
MAC3 ES cells
ES cells are pluripotential
ES cells provide the system where we are able to modify the genome
What if we stick cells back into an embryo?
iPS cells from black mouse
ES cells from white mouse
Return to pseudopregnant fosters
This mouse works! But can we get a whole mouse from iPS cells?
Human dermofibroblasts (HDF’s)
Transformed HDF’s making colony just like an ES cell colony