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ACADEMIA SINICA IBMS

ACADEMIA SINICA IBMS. 嚴仲陽 美國德州貝勒醫學院 博士. Specialty: Molecular & Cell Biology Hematopoiesis Apoptosis Regulation. Method Yeast Two-hybrid. Material. Yeast strain : L40 ( -his 、 -trp 、 -leu 、 -ade ) Plasmid : pBTM116 & pACT2 Competent Cell : HB101 Yeast DNA purification kit

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ACADEMIA SINICA IBMS

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  1. ACADEMIA SINICAIBMS 嚴仲陽 美國德州貝勒醫學院 博士 Specialty: Molecular & Cell BiologyHematopoiesisApoptosis Regulation

  2. MethodYeast Two-hybrid

  3. Material • Yeast strain:L40 (-his、-trp、-leu、-ade) • Plasmid:pBTM116 & pACT2 • Competent Cell:HB101 • Yeast DNA purification kit • E coli. DNA purification kit

  4. Result • No Result ……

  5. Normal Transcription

  6. Yeast Two-hybrid Transcription

  7. Plasmid construction

  8. Bait & Prey

  9. Bait-pBTM116

  10. Prey-pACT2

  11. Yeast Two-Hybrid

  12. Transformation & Assay-Trp, -Leu, -His

  13. β-galactosidase assay

  14. gs3955 & library • Yeast Transformation • Pick colonies • Selection three times • β-galactosidase assay • Yeast DNA purification • Transform to HB101 • Bgl Ⅱ check • Retransform to yeast • Sequencing

  15. My summer vacation • Experiment • Travel • Camp

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