Lab. 21 Sand-fly &Flies

1. Lab. 21Sand-fly &Flies

2. Sand fly (Al-Hermis)

3. Phylum: Arthropoda Class: Insecta Order: Diptera Family: Phlebotomidae

4. Phlebotomuspapatasi: it is the most dominant species. P. sergenti: an importantspecies. P.Alexandri Sergentomyiabagdadi&S. dentata They are important spp. to keep the infection among rodents. Lutzomayia it is important in the New World, not Old World.

5. Phlebotomus (Sandfly) adult Female & male • with dark colored hair. • Sandy colored. • Have piercing- sucking mouth parts. • Eyes are large and filling the head. • Fragile insects, weak fliers, hump (jump). • Long legs, wings stand up and never fold on abdomen. • Adult Phlebotomus papatasi ♀: with hair, dark colored may be the most important vector for Leishmania and papatasi fever.

6. Adult sausage shape, mosaic, yellow color, never found in nature Pupa Egg 1 4 4rth stage larvae, with black capsule-like head and spins on body & 2 or 4 hairs at the end. 2 4 Larval Instars 3

7. Adult Phlebotomuspapatasi♂: with outer genitalia, was seen at the end of body…with lower claspers and aedigus.

8. Egg of sand fly: sausage shape, mosaic, yellow color, never found in nature…7-10 days. • 4rth stage larvae, with black capsule-like head and spins on body & 2 or 4 hairs at the end. • Pupa with old exuvae at end.

9. Resting position Feeding female Female Male

10. Sand fly larva • black capsule- • like head • spins on body • 2 or 4 hairs at • the end

11. Lab 20 Common fly & myiasis

12. Phylum: Arthropoda Class: Insecta Order: Diptera Suborder: Cyclorhapha

13. Complete metamorphosis • 5-6 days from eggs to egg-lying Larva Eggs Pupa Adult 25-30 days

14. Family Muscidae: The housefly family, Medium to small size. Gray to shiny colors. Wings broad reduced veins.subfamily: Muscinae(House fly) Gray color,Lapping mouth parts. Lower lip modified with spongy wide tip. Do not bit.Lived on filthy, decayed, semi fluid. Ex: houseflies; Muscadomestica: annoying, mechanical transmission. Larvae are maggot type (pointed front & wide end). Musca domestica

15. Spongy mouth part of House fly

16. Family Muscidae: Subfamily: Stomoxyinae 1. Stable fly Stomoxiscalcitrans • Medium size • modified mouthparts for • biting and blood sucking. • Live in houses, gardens, • parks. • Vector of surra disease • Trypanosoma eyeansi on • animals… • Reproduction by • oviparous larvae like • these of house flies. Stomoxiscalcitrans

17. Family Muscidae: Subfamily: Stomoxyinae : 2. Glossina (tsetse fly) • Found only in Africa, • Vector of African sleeping • sickness. • Reproduce by viviparous. • give birth to larvae very • mature soon become pupa • in soil. • Parasitic on blood… give • birth to one larva at time, • every needs or so.

18. Order: Diptera Family: SimuliidaeBlack fly (Simulium)Onchocercavolvulus causes onchocerciasis or "river blindness"

19. Black fly (Simulium)

20. Myiasis

21. Myiasis: • Invasion of living tissues & organs by • larva of advanced flies…. • Larvae live on host, pupa in soil, • many of them are viviparous (give birth • to larvae). • Larvae are maggot or bot which stubby, • two ends are recusal.

22. Myiasis producing families: • Family:Muscidae…….common house flies • False Intestinal myiasis. Musca domestica

23. 2. Family: Sarcophagidae (flesh fly) True Intestinal myiasis. Large, less board like, gray in color.

24. 3. Family: Calliphoridae. • shining colors. Ex: • Hypodermaovinus, • 2. Dermatobiahominis • 3. Cordylobiaantheropophaga • Tumbu fly • 4. Chrysomyiabezziani Dermal myiasis: a.Creeping irretation: Hypoderma spp. Dermatobiahominis Dermatobia hominis

25. 3. Family: Calliphoridae. • shining colors. Ex: • Hypodermaovinus, • 2. Dermatobiahominis • 3. Cordylobia • antheropophaga • Tumbu fly • 4. Chrysomyiabezziani Chrysomyia bezziani Traumatic myiasis: Chrysomyiabezziana

26. 4. Family: Oesteridae. naghaf flies. Ex: Oesterus ovis Nasophayngeal myiasis: Oesteris ovis, nasopharynx in sheep, ophthalmomyiasis in man .

27. Bot larvae

28. Maggot larva