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oVM246. 3’ss. oVM245. Exon 3. oVM244. K8 β intron. …acacaagacagcugcagcag GUAUAGACGGGAAACAGGUGUCUAUCUUGGCCGGCUGGUUACUCAAAUGGGAACAAUGGCGCCACCUUGCUGUCUUUGUAG gcauuagaagaaaaggaugc…. oVM243. Exon 2. oVM242. STOP. 5’ss. oVM241. Figure S1. K8 β. 1. 2. 3. 4. RBM15. -. -. +. +. -.

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Presentation Transcript
slide1

oVM246

3’ss

oVM245

Exon 3

oVM244

K8β intron

…acacaagacagcugcagcagGUAUAGACGGGAAACAGGUGUCUAUCUUGGCCGGCUGGUUACUCAAAUGGGAACAAUGGCGCCACCUUGCUGUCUUUGUAGgcauuagaagaaaaggaugc…

oVM243

Exon 2

oVM242

STOP

5’ss

oVM241

Figure S1

slide2

K8β

1

2

3

4

RBM15

-

-

+

+

-

ORF57

-

+

+

K8

+

+

+

+

C

N

C

N

RNA

C

N

C

N

-

K8

-

GAPDH

U6

Figure S2

slide3

A

BCBL-1

0

6

12

24

32

56

VA (h)

SRSF3

RBM15

- full

ORF57

- cleaved

β-tubulin

SRSF3/RBM15 (%)

1

4

47

878

2220

2478

B

HEK293

1000

ORF57 DNA (ng)

100

500

0

SRSF3

RBM15

- full

- cleaved

ORF57

β-tubulin

Figure S3

slide4

Supplementary Table S1: Peptides and corresponding proteins associated with KSHV ORF57 in THE absence (Fig 2A, sample 1) or presence (Fig 2A, sample 2) of ectopically expressed RBM15. The peptides and proteins were identified with LC-MS/MS.

slide5

SUPPLEMENTARY TABLE S2. Biotinylated RNA oligos from the KSHV K8β intron region and

the HPV16 ESE used in RNA pulldowns. Underlined are point mutations.

slide6

Supplementary Table S3: Primers used for RT-PCR and Northern Blotting.

T7, T7 promoter; U1bs, U1 binding site

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