Duplex qPCR for BCR-ABL1 MRD. Gareth Gerrard [email protected] Leukaemia MRD Unit, Centre for Haematology, Imperial College, Hammersmith Hospital, London. EAC MRD qPCR. The Hammersmith Problem:. ABL. BCR-ABL. Duplicates: 36 Samples. Triplicates: 22 Samples.
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Duplicates: 36 Samples
Triplicates: 22 Samples
Average Daily Run: 1 ABL1 plate & 2 BCR-ABL1 plates
= 44 Samples per day fully quantitated
Triplicates: 24 Samples
Average Daily Run: 2 Duplex plates
= 48 Samples fully quantitated
BCR-ABL1 (BCR ex13 – ABL1 ex2)
ABL1 (ABL1 ex3 – ABL1 ex4)
Optimised to achieve equivalent Cts for both targets