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The Effect of Ginkgo Biloba Amyloid , Freeze Dried Black Raspberries and Freeze Dried Blueberries on the supression of Amaloid B Protein in C. elegans. Roger Coleman. Need.

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Roger coleman

The Effect of Ginkgo BilobaAmyloid, Freeze Dried Black Raspberries and Freeze Dried Blueberries on the supression of Amaloid B Proteinin C. elegans

Roger Coleman


Need

  • Alzheimer’s disease costs patients, Medicare or other types of insurance agency's up to $148 billion dollars a year and for patients 65 and older it will triple healthcare costs. In America alone 5.3 million people live with Alzheimer's. Alzheimer's is also the seventh leading cause of death.


Need

Chart 1 shows statistics and future predictions of Alzheimer's

disease

http://www.alzalliance.org/images/Forcast%20of%20Alzheimer%27s%20disease%20chart.gif


Knowledge base
Knowledge Base

  • Figure 1 shows C.elegans

http://shirleywho.files.wordpress.com/2008/09/c-elegans_esa.jpg


Knowledge base1
Knowledge Base

Figure 2 shows the lifecycle of c. elegans


Knowledge base2
Knowledge Base

Picture 3 shoes a cross reference of a normal brain and a Alzheimer‘s infected brain.

http://www.realage.com/health_guides/Alzheimer/img%5CLivingWithAlzheimers_artv2.jpg


Knowledge base3
Knowledge Base

Figure 4shows an amyloid b protein

http://64.202.120.86/upload/image/news/getting-out-of-the-loop-of-alzheimer-s-disease/amyloid-protein-loop.jpg


Knowledge base4
Knowledge Base

Chart 2 shows amaloid b protein product


Knowledge base5
Knowledge Base

This picture shows other foods similar to Ginkgo Biloba that contain flavonoids.

http://www.cyberline.com/juice/berrybg.JPG


Literature review
Literature Review

  • (Wu et, al, 2006)

This shows the difference between a untreated amaloid b compared to a Egb-fed


Literature review1
Literature Review

  • Ginko Biloba is well known for supporting brain function. (Colman 2001)

  • Journal of the American Medical Association found that 120 mg of ginko can improve brain function.

http://www.made-in-china.com/image/2f0j00DedaEMtgOzqoM/Ginkgo-Biloba-Leaf-Extract-CP2005-24-6-HPLC-.jpg


Literature review2
Literature Review

  • (Ercoli, 2006 et al) found that significant improvement was shown by verbal and memory impaired patients using the ginko berry leaf

Figure 5 shows the plant of Ginkgo biloba

http://www.herbalextractok.com/HerbalExtract/Ginkgo_Biloba_extract.jpg


Purpose
Purpose

To determine if ginkgo biloba amyloid, freeze dried black raspberries and freeze dried blueberries has an effect on Alzheimer dieses in C. elegans.

Hypothesis

Null hypothesis is that no effect will occur when ginkgo biloba amyloid, freeze dried black raspberries and freeze dried blueberries ware added.

The Alternative hypothesis is that ginkgo biloba amyloid, freeze dried black raspberries and freeze dried blueberries


Methodology
Methodology

The Effect of Ginkgo Biloba Amyloid, Freeze Dried Black Raspberries and Freeze Dried Blueberries on the suppression of Amaloid B in C. elegans

C. Elegnas with amaloid b and ginkgo biblia solution (Experimental)

C. Elegans (CL2006) strainf and given a 10 % solution of Ginkgo Biloba N=?

C. Elegans ( CL2006 strain) will be given a 10% solution of Freeze dried Black raspberries

N=?

C. Elegans (CL2006 strain) will be given a 10 % solution of Freeze Dried Blueberries N= ?

C. Elegnas ( Cl 2006 strain with no solutions N= ?

(Control)

C. Elegans will be fed with 100 ul spots of e. coli (OP5O) on nematode growth medium and c. elegans will be cultured a 20 degrees C

Results will be taken from visual analysis of C. elegans under a microscope.


Protocols for measurement
Protocols for Measurement

  • Grown on NGM and C. elegans (control and CL2006) will be fed with E. Coli (OP5O) with 100 ul spots.

    Ginkgo Biloba Amyloid, Freeze Dried Black Raspberries and Freeze Dried Blueberries will be given in the NGM in a 10 % solution to both the CL2006 strain and the control strain


Protocols for measurement cont
Protocols for Measurement (cont)

  • All solutions will be given for one day at a time and analysis will be analyzed after 2 days.

  • Analysis will be compared ( under a microscope) to the number of normal and the number of paralyzed c. elegans


Do ability
Do- ability

  • C. elegans and chemicals are easy to buy

  • Much of the equipment is already in the lab

  • Chemicals are easily applied to C. elegans




Bibliography
Bibliography

  • Works Cited

  • Hancock, Robert D. "Berry fruit as ?superfood?:." Scottish Crop Research Group 54 (2007). Http://www.iob.org/userfiles/File/biologist_archive/Biol_54_2_Hancock.pdf.

  • Luo, Yuan. "Inhibition of amyloid -b aggregation and caspase- 3 activation by the Ginkgo biloba." Departments of Biological Sciences and Chemistry and Biochemistry (2002).

  • Wilson, Mark A. "Blueberry polyphenols increase lifespan and thermotolerance in Caenorhabditis elegans." PubMed Central (2006).

  • Wilson, Mark. "Bluebrry polyphenols increase the lifespan of c. elegans." Blackwell Publishing Ltd (2006).

  • Wu. "Egb reduces ab specific pathological behaviors are suppressed by ginkgo biloba extract Egb 761 in caenorhabditis elegans." (2006).

  • Yang, Huan. "The cytokine activity of HMGB1." Laboratory of Biomedical Science.


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