Forest health initiative a model for rapid response to emerging pests
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Forest Health Initiative: A Model for Rapid Response to Emerging Pests. C. Dana Nelson, Project Leader/Research Geneticist [email protected] Southern Research Station Southern Institute of Forest Genetics Harrison Experimental Forest Saucier, MS. Forest Health Crisis.

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Forest health initiative a model for rapid response to emerging pests

Forest Health Initiative:A Model for Rapid Response to Emerging Pests

C. Dana Nelson, Project Leader/Research Geneticist

[email protected]

Southern Research Station

Southern Institute of Forest Genetics

Harrison Experimental Forest

Saucier, MS

Kentucky/Tennessee/SRS Stateline Meeting


Forest health crisis
Forest Health Crisis

  • Invasive pests, climate change

    • New approaches and tools required to slow decline and recover health

  • Forest Health Initiative developed to test a hypothesis:

    • that a coordinated effort in biotechnology research will lead to resistant trees capable of restoring a species in a relevant time frame

      • American chestnut selected as test case

      • www.foresthealthinitiative.org

Kentucky/Tennessee/SRS Stateline Meeting


Forest health initiative fhi
Forest Health Initiative (FHI)

  • Key components of FHI hypothesis:

    • Coordinated effort

      • Steering Committee, Social/Environmental Group, Science Advisory Committee, PIs, Cooperating Scientists and their research teams

    • Biotechnology research

      • Biological Sciences effort– genetics, molecular biology, pathology

    • Resistant trees in Relevant time

      • ensure blight resistance through seeds from adapted and diverse American chestnuts

      • 3 years to a ‘plantable tree’

        • tangible progress w/array of promising trees going to field tests

        • infrastructure/know-how-- to plant and grow trees in reintroduction/restoration

Kentucky/Tennessee/SRS Stateline Meeting


Start

0

CmSI0050

26984

CmSI0660

1585392

CmSI0532

2341908

CmSI0531

2776209

R04_0775

3077960

CmSI0551

4712728

CmSI0437

6041717

Cmp0028

7163814

End

7403370

Integrated mapping necessary for finding

candidate resistance genes

Genetic mapping

QTL mapping

Physical mapping

BAC contigs

Genome Sequence contigs/scaffolds

Kentucky/Tennessee/SRS Stateline Meeting

CD Nelson, USFS; M. Staton, Clemson; J Carlson, Penn State


Field tests of genetic materials are

important for advancing program

SG2-3

Chestnut crosses at age 2

EP155

Inoculations at age 6

Measure canker sizes,

late summer, age 6

Kentucky/Tennessee/SRS Stateline Meeting

The American Chestnut Foundation


Gene transformation transgenic plant production
Gene Transformation – Transgenic Plant Production

Agrobacterium (AGL1) infection of chestnut PEMS

TG somatic seedlings in pots

Selection of transgenic (TG) colonies with Geneticin

TG somatic shoots in vitro

Air-lift bioreactors make cells for transformation every 2 wk

TG events in somatic embryogenesis (SE) production

TG events grown in flasks

TG events grown on plates

somatic embryos harvested

Kentucky/Tennessee/SRS Stateline Meeting

S. Merkle, Univ. Georgia


Early leaf assay
Early Leaf Assay

American

Necrotic length (mm)

Chinese

American

Chinese

Error bars = 1 SEM

T-test: P<.0001

  • Combined data from 5 experiments (48-52 inocs per leaf type)

Chinese chestnut leaves consistently show less necrosis than American; same pattern seen in field inoculations

Kentucky/Tennessee/SRS Stateline Meeting

W. Powell, SUNY- ESF


Field tests evaluating improvements e g 640 transgenic trees 534 controls
Field Tests, evaluating improvements(e.g., 640 transgenic trees & 534 controls)

Kentucky/Tennessee/SRS Stateline Meeting

W. Powell, SUNY- ESF


Fhi conclusions
FHI Conclusions

  • FHI hypothesis is tentatively accepted

    • ‘plantable trees’ have been achieved in short period

    • know-how & infrastructure developed

  • Continuing work will

    • evaluate the new materials under field conditions

      • advance winners to additional field tests

    • apply mapping resources to breeding programs

  • Adapt FHI to new case

    • e.g., thousand cankers disease

Kentucky/Tennessee/SRS Stateline Meeting


Thousand cankers disease basic biological sciences needs
Thousand Cankers Diseasebasic biological sciences needs

  • Screening methods for assaying resistance

    • insect vector and/or fungus pathogen

  • Genetics of host resistance/susceptibility

  • Markers and genes for resistance

  • Biotech tools for engineering resistance

  • Breeding populations for deploying resistance

    • Cooperators with trees of interest and land for field testing

Kentucky/Tennessee/SRS Stateline Meeting


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