The mechanism of HCV extrahepatic infection. Michael M.C. Lai Institute of Molecular Biology Academia Sinica. Sept. 21, 2012 at Wuhan. Clinical manifestations of HCV. Evidence that PBMC infection by HCV is causally linked to B cell lymphoma.
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The mechanism of HCV extrahepatic infection
Michael M.C. Lai
Institute of Molecular Biology
Sept. 21, 2012 at Wuhan
A lymphotropic HCV (HCV-SB) isolated from a B-cell lymphoma
Hecker, E. & Ott, M. 2011. Ubique ties between hepatitis C virus replication and intracellular lipids. Cell. 22(6).243
Molecular mechanism of viral lympho- vs hepatotropism
T7 in vitro
into Huh7.5 and Raji
SB virus RNA, but not JFH-1 Virus, Replicates in Raji Cells
Chimera Virus containing E1-E2 of SB Strain Produces High Levels of HCV RNA in Raji Cells
Infection of target cells
GFP orluciferase expression
Study virus entry, receptors, host range, etc.
HCV Pseudoparticle System (HCVpp)
A retrovirus reporter virus with HCV envelope proteins
GFP or Luciferase
Infectivity of HCV Pseudo-particles is Determined
by E1-E2 of SB Strain in Raji Cells
FACS for GFP reporter with HCVpp at 72 h p.i
Normalized for p24 HIV core antigen
Mutation frequencies of p53 and VH
in HCV-infected human B cell lines
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