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http://goo.gl/B6Tqa. PMID: 22137970. Транскрипция. Три транскрипта: C HSP1 – в D-loop C HSP2 – upstream 5’- конца 12 S rRNA (частота инициации в 20 раз ↓ ) С LSP – в D-loop. PMID: 17189185. В транскрипции участвуют:. POLRMT TFAM (h-mtTFA –transcription factor A)

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Goo gl b6tqa

http://goo.gl/B6Tqa


Goo gl b6tqa

PMID: 22137970

  • :

  • C HSP1 D-loop

  • C HSP2 upstream 5- 12 S rRNA ( 20 )

  • LSP D-loop


Goo gl b6tqa

PMID: 17189185

:

  • POLRMT

  • TFAM (h-mtTFAtranscription factor A)

  • TFBM1 (h-mtTFB1)

  • TFBM2 (h-mtTFB2) - POLRMT

  • MTERF1 (mitochondrial termination factor)

  • TFEM (transcriptional elongation factor mitochondrial) - POLRMT


Goo gl b6tqa

PMID: 22551784

- POLRMT

TFAM :TFBM1TFBM2


Goo gl b6tqa

  • TFAM .

  • TFBM1 (h-mtTFB1) TFBM2 (h-mtTFB2) -, 3- 12S p. - , .

  • , .

  • TFBM1 TFBM2 .

TFAM

PMID:

17189185


Goo gl b6tqa

PMID: 22642575

TFB1M TFB2M 936 937 12S rRNA

  • TFB1M:

  • 12S rRNA

  • .


Goo gl b6tqa

  • TFB M1 2 :

  • ( )

  • - (MT)

  • - in vitro.

  • TFB M1 iRNA , => TFB M1

TFB M1 .


Goo gl b6tqa

  • TFBM2:

  • -

  • :

  • iRNA 2-8 .

  • 2 :

  • ,

  • , - , .


Goo gl b6tqa

  • TFBM1 (h-mtTFB1) TFBM2 (h-mtTFB2) -, 3- 12S p

  • TFBM1 TFBM2 , POLRMT

  • TFBM1 ,

  • TFBM2 .

  • TFBM1 12S p

  • TFBM2


Goo gl b6tqa

PMID: 22465614

TFAM .

  • TFAM : 77%

  • 246 , 25

  • 23% + .


Goo gl b6tqa

PMID: 22465614

  • TFAM:

  • Mitochondrial Targeting Sequence (MTS)

  • HMG (High mobility group) box I

  • linker

  • HMG box 2

  • C-terminal tail -


Goo gl b6tqa

TFAM (transcription factor A).

HMG (High mobility group) box:

3 2 , L-.

, .

PMID:22037171


Goo gl b6tqa

TFAM , POLRMT : LSP HSP1, upstream .

TFBM1/POLRMT TFBM2/POLRMT in vitro.


Goo gl b6tqa

PMID:22037171

TFAM : TFAM , .

TFAM LSP HSP1, , LSP , HSP1. -.

, , , -.

LSP .

TFAM LSP, HSP1.


Goo gl b6tqa

PMID:22037171

TFAM ( -), LSP, , LSP=>TFAM .


Goo gl b6tqa

PMID:22465614

TFAM:

TFAM , . -.


Goo gl b6tqa

PMID:22465614

  • TFAM:

  • . : ?

  • , -.

  • LSP .


Goo gl b6tqa

PMID:22465614

  • TFAM :

  • TFAM: :

  • TFAM, TFAM: .

  • TFAM :

  • (+) TFAM


Goo gl b6tqa

  • TFAM

  • 2 :

  • TFAM LSP .

  • POLRMT LSP . TFAM LSP , HSP1.

  • TFAM 1 :

  • 50 HeLa

  • 35 HEK 293

  • LSP 85%, HSP1 35%.


Goo gl b6tqa

2. TFAM

TFAM - TFAM , .

TFAM 1 : 900-1600 =>TFAM 35-36 .

TFAM : TFAM , .

TFAM Lon. : , , .


Goo gl b6tqa

Basal transcriptional activity - no TFAM

PMID: 22465614


Goo gl b6tqa

PMID:23201127

TFAM

  • Lon TFAM: :

  • Lon TFAM

  • Lon TFAM


Goo gl b6tqa

TFAM.

PMID:23201127

TFAM HMG box 1 (HMG1) cAMP- PKA. TFAM .

TFAM (DNA-free TFAM) Lon.


Goo gl b6tqa

  • TFAM ,

  • TFAM

  • TFAM , POLRMT HSP1 LSP

  • TFAM LSP, HSP1

  • TFAM

  • TFAM : , TFAM,

  • TFAM


Goo gl b6tqa

PMID: 22137970

  • 1 tRNA Leu

  • 2 -

  • L -

  • mTERF1.


Goo gl b6tqa

  • MTERF1(mitochondrial termination factor) 39

  • 8 Mterf -

PMID: 20550934

(D).


Goo gl b6tqa

PMID: 20550934

MTERF . 25. -, .

L- - , MTERF1: R162, F234, Y288.


Goo gl b6tqa

PMID: 20550934

- .

MTERF1 ( R162, F234, Y288) (), => 1 , .

R162, F234, Y288, R162 => MTERF1 .


Goo gl b6tqa

MTERF1 : .

?


Goo gl b6tqa

MTERF1 , 3 .

MTERF1 ( R162, F234, Y288) wtMTERF1, wtMTERF1 => .

, .


Goo gl b6tqa

- 5 Arg MTERF1 .

Arg, , , MTERF1 .

MTERF Arg .


Goo gl b6tqa

MTERF1 .

, , .

PMID: 20550934


Goo gl b6tqa

MTERF1 in vitro

PMID: 22137970

HSP1, in vitro

: MTERF1 HSP1 16S . POLRMT HSP1, .

, MTERF1 HSP2.


Goo gl b6tqa

PMID: 22137970

In vitro Leu MTERF1 : .. 1 L. LSP .

2 upstream Phe. . 2 , .


Goo gl b6tqa

PMID:21326908

MTERF1 E.coli

Tus .


Goo gl b6tqa

POLRMT G-

7: - , .

, HSP2. , MTERF1 - .


Goo gl b6tqa

G-


Goo gl b6tqa

  • MTERF1

  • A3243G 80% MELAS (Mitochondrial Encephalomyopathy, Lactic Acidosis, Strokelike Episodes). HSP, Leu. LSP .

  • G3249G Kearns-Sayre

  • G3242A


Goo gl b6tqa

  • MTERF1

  • MTERF1 ,

  • MTERF1 5 Arg

  • MTERF1 in vitro


Goo gl b6tqa

  • MTERF

  • MTERF1 MTERF2

  • MTERF3 MTERF4 , ( )

  • MTERF1-3 , MTERF4 .


Goo gl b6tqa

  • MTERF2

  • : : .

  • , => MTERF2 .

  • => MTERF2


Goo gl b6tqa

PMID: 20430012

MTERF3

MTERF1. MTERF3 1 Arg , , .

: , .. .

MTERF3 .


Goo gl b6tqa

PMID: 22642575

MTERF4

. , .

16S , 16S .

, .


Goo gl b6tqa

PMID:22120174

  • POLRMT- -, -

  • TFAM (h-mtTFAtranscription factor A) .

  • TFBM1 (h-mtTFB1) - (MT), ,

  • TFBM2 (h-mtTFB2) , POLRMT, - (MT)

  • TFEM (transcriptional elongation factor mitochondrial) - POLRMT

  • MTERF1 (mitochondrial termination factor)


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