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Effects of Baicalin on the gene expression of surfactant protein A (SP-A) in lung adenocarcinoma cell line H441
Surfactant protein A (SP-A) is the most abundant surfactant protein component of pulmonary surfactant, constituting about 50% of the total surfactant protein. SP-A is a material secreted by the alveolar type II cell that reduces surface tension at the alveolar air-liquid interface. It is an important role on the function of the alveolus.Respiratory Distress Syndrome (RDS) is major cause of morbidity in preterm neonates, which results from a deficiency of pulmonary surfactant. Maternal glucocorticoid (ex: Dexamethasone) and exogenous surfactant (ex: Surrata and Exosurf) therapy decreased the incidence of death and RDS, but the treatments glucocorticoid and exogenous surfactant have an adverse effect on the brain development of the premature infant.Analysis of SP-A gene expression using RT-PCR, cDNA subtraction, Western Blotting and 2D in this study. The result of H441 cell culture assayed with RT-PCR and Western blot showed that the gene expression of SP-A was increased when treating with Baicalin (one of the major components of Scutellariae Radix) in time and dosage model. The maximal gene expression of SP-A is exhibited at 150 nM baicalin treated for 48 hours when cDNA subtraction analysis performed. The result shown that treated with baicalin increased copper-zinc superoxide dismutase and cytochrome C oxidase expression and transducin-like enhancer of split 1, which resulted in the elevation of ATP level in the cell, the SP-A secretion thereby increased consistently.