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Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes ( Solanum tuberosum)

Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes ( Solanum tuberosum). Shannon Engelman 2007. University of MN Department of Horticulture. 2,4-dichlorophenoxyacetic acid (2,4-D). http://files.agrichem.net/agrbv/artinfo/foto.org/2,4,%20d%20Amine%20a.JPG. Background.

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Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes ( Solanum tuberosum)

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  1. Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon Engelman 2007

  2. University of MNDepartment of Horticulture

  3. 2,4-dichlorophenoxyacetic acid (2,4-D) http://files.agrichem.net/agrbv/artinfo/foto.org/2,4,%20d%20Amine%20a.JPG

  4. Background • Light intensity and hue angle decreases (Rosen, et al.)

  5. Components of Color • Light intensity • Chroma (saturation) • Hue angle www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf

  6. Components of Color • Light intensity • Chroma (saturation) • Hue angle www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf

  7. Components of Color • Light intensity • Chroma (saturation) • Hue angle www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf

  8. Components of Color • Light intensity • Chroma (saturation) • Hue angle www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf

  9. Background • Light intensity and hue angle decreases (Rosen, et al.) • Coloration affects sales (Fults, et al.)

  10. Background • Light intensity and hue angle decreases (Rosen, et al.) • Coloration affects sales (Fults, et al.) • 2,4-D harmful, LD50 of 1500 ppm (MSDS found at http://fscimage.fishersci.com/msds/96919.htm)

  11. Goal • To determine visual color, anthocyanin accumulation, and UFGT accumulation differences

  12. Anthocyanin Biosynthetic Pathway LDOX UFGT leucocyanidinanthocyanidinanthocyanin 3-glucoside (Keifenheim, et al.)

  13. Hypotheses With 2,4-D application: • Increase in UFGT accumulation

  14. Hypotheses With 2,4-D application: • Increase in UFGT accumulation • Change in color (Rosen, et al.)

  15. Hypotheses With 2,4-D application: • Increase in UFGT accumulation • Change in color (Rosen, et al.) • Increase in anthocyanin accumulation

  16. Methods

  17. Colorimetry http://www.recreonics.com/images3/pocket_colorimeter.gif

  18. Components of Color • Light intensity • Chroma (saturation) • Hue angle www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf

  19. Anthocyanin Accumulation

  20. Quantifying UFGT RNA

  21. Quantifying UFGT RNA cDNA reverse transcription

  22. Quantifying UFGT Amplified UFGT RNA cDNA reverse transcription qPCR

  23. Results S S

  24. Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field Study Greenhouse Study

  25. Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field Study Greenhouse Study

  26. Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field Study Greenhouse Study

  27. Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field Study Greenhouse Study

  28. Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field Study Greenhouse Study

  29. UFGT Field Data

  30. UFGT Greenhouse Data

  31. Conclusion • Visual color significantly changed (light intensity, hue angle)

  32. Conclusion • Visual color significantly changed (light intensity, hue angle) • Anthocyanin accumulation unchanged

  33. Conclusion • Visual color significantly changed (light intensity, hue angle) • Anthocyanin accumulation unchanged • UFGT accumulation decreased

  34. Future Work • Look at petunidin (purple) vs. pelargonidin (red) anthocyanins

  35. Acknowledgements • Dr. Cindy Tong • Harpartap Mann • Dr. Carl Rosen • Ms. Fruen • Team Research

  36. Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon Engelman 2007

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