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Hershey's work on T2 phage PowerPoint PPT Presentation


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Hershey's work on T2 phage. DNA, not protein is hereditary substance. CONTENT. 1.Hershey History. 2. What is T2??. 3. Infective Cycle. 4. Hershey Chase Experiment. 5. Conclusion. Alfred Day Hershey. His best known experiment is Hershey Chase experiment

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Hershey's work on T2 phage

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Hershey's work on T2 phage

DNA, not protein is hereditary substance


CONTENT

1.Hershey History

2. What is T2??

3. Infective Cycle

4. Hershey Chase Experiment

5. Conclusion


Alfred Day Hershey

  • His best known experiment is Hershey Chase experiment

  • Concerned the replication of genetic structure of viruses

4 Dec. 1908--- 22 May 1997


1934 -1950Teaching and research at the Department of Bacteriology1936Held an instructorship in bacteriology and immunology 1936 -1939Work together with Professor J. J. Bronfenbrenner. 1938Became assistant professor 1942Became associate professor


What is T2 ?

  • The T2 phage is a strongly toxic bacteriophage and tadpole-shaped virus

  • small virus that infect only bacteria

  • Contain 50% DNA and 50% protein


Infect E-coli

  • Contains linear double-stranded DNA


tail fibers of the phage attach to the bacterial cell to initiate infection


Protein coat of the T2 carries the organ of

attachment to bacteria.


The infective cycle of T2 in E.coil

  • Virus attach to the surface of their host cell(a)


  • Inject the DNA into the cell(b)


  • DNA accumulate,other genes are transcribed and translated(C)


  • DNA and protein are assembled into complete virus (d)


  • The cell breaks and releases its content of virus(e)


Hershey ChaseExperiment

  • Radioactive Sulphur (35S)

    non-radioative DNA?

  • Radioactive Phosphorous(35P)

    radioactive DNA?


Conclusion

DNA is the

GENTIC MARTERIAL!!!


~THE END~


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