Hookworms. Associated prof. Tian. Background. 1. Hookworms are the voracious blood feeders of the nematode world Hookworms parasitize more than 900 million people worldwide. 2. Ancylostomiasis is second only to ascariasis in infections by parasitic worms.
Associated prof. Tian
1. Hookworms are thevoracious blood feedersof the nematode world
Hookworms parasitize more than 900 million people worldwide.
2. Ancylostomiasis is second only to ascariasis in infections by parasitic worms.
3. There are two species of hookworms of medical importance
Ancylostoma duodenale: - Africa, India, China and South East Asia (Asia hookworms)
Necator americanus: - First reported in Brazil, then Texas, but since found in Africa, India and South East Asia.
The adult parasites are small cylindrical worms, 0.5 - 1.5cm long (Ancylostoma duodenale being slightly larger than Necator americanus ).
buccal capsule(mouth capsule)
The anterior end of the parasites are formed into a buccal capsule
The posterior end of the male worm is equip with a characteristic copulatory bursa
Copulatory bursa of male of A.duodenale
Copulatory bursa of male of N.americanus
Comparison of Hookworms
Size (lenth) about 10 mm slight larger
Shape “ S ” “ C ”
buccal capsule a pair of two pair of
cutting plates curved teeth
Size 57-76 µm by 35-47 µm
Oval or ellipsoidal shape Thin shell, colorless. The eggs usually contain 4-8 cells in feces
A: Hookworm egg, advanced cleavage (iodine).B: Embryonated hookworm egg.
Are passed in the stool
warm moist shaded soil
Contact host and penetrate the skin into the body
Migrant by lungs
The route of larvae migrant
Skin lungs trachea
Oesophagus stomach intestine
Soil polluted with human excreta is commonly responsible for exposure to infection with human hookworms
Individuals become infected, usually by walking bare footed across contaminated soil
Penetration of the intact skin by filariform larvae of hookworm, on coming in contact with fecal polluted soil.
A, B: Hookworm rhabditiform larva (wet preparation).
A, B: Hookworm filariform larva (wet preparation).
A, B, C: Hookworm filariform larva (A and B, wet preparations; C, iodine). Larva tail is depicted in Figures B and C.
Pathogenesis and clinical manifestation
The larva of a hookworm is visible in the center of this photograph
(2) Pulmonary phase:by hookworm larvae
Local haemorrhaging, pneumonitis
Symptoms in respiratory system
cause a cough and a sore throat.
(3) Intestinal phase:By adult worm
A)the intestinal mucosa are damaged
Protein and iron in-take is insufficient
Hypochromic microcytic anemia
a)Ingestion of the blood by the worm
b)Seepage of the blood around the site of attachment of the worm
c)Oozing of the blood from the burrowed site previously attached by the worm
This is a photograph of the hookworm's appearance against the lining of the intestine (intestinal mucosa).
Clinical features of hookworm disease
Local erythema, macules, papules (ground itch)
Cutaneous invasion and subcutaneous migration of larva
Bronchitis, pneumonitis and, sometimes, eosinophilia
Migration of larvae through lung, bronchi, and trachea
Anorexia, epigastric pain and gastro-intestinal hemorrhage
Attachment of adult worms and injury to upper intestinal mucosa
Iron deficiency, anemia, hypoproteinemia, edema, cardiac failure
Intestinal blood loss
It includes parasitic diagnosis and immunodiagnosis
l) Microscopy Direct smear examination of feces is adequate to detect moderate or severe infections.
2) Concentration Concentration of stool by formalin-ether or simple salt floatation stool is essential to detect light hookworm infection.
3) Third-stage larvae in the fecal culture
Hookworm diseases is widely epidemic parasitic disease in the world. Hookworm distribute these areas between northern latitude 45○ to southern latitude 30○. A.duodenale is chiefly found in tropic areas and subtropic areas, N.americanus is commonly found in warm zone.
Distribution of Hookworm in china
Hookworm: World Distribution
Reservoir, source and transimission
Human is the only reservoir of infection
Human feces is the only source of infection
Prevention and control
l ) Sanitary disposal of human feces;
2) Treatment of infected persons;
3) Health education with improved use of sanitary latrines and use of foot wears
Treatment of hookworm infection consists of
a) treatment of worm infection by anthelmintics such as mebendazole;
b)treatment of anemia.
Trichinosis is related to the quality of pork and consumption of poorly cooked meat. Autopsy surveys indicate about 2 percent of the population is infected.
Adult worm The adult worms are very small and slender with slightly tapered anterior ends, white and just to the naked eye. female 2.2 mm in length, males 1.2 mm;
Its pharynx is one third or half of worm body long, and posterior part of pharynx consists of a column of cells called of stichocytes.
The cyst are found in skeletal muscle commonly, its size is about 0.25~0.5
Larvae in Muscle Section
Larvae in Muscle Press
Larvae of Trichinella, freed from their cysts, typically coiled; length: 0.8 to 1.0 mm.
Trichinella spiralis: Electron microscopy
Into skeletal via bloodstream
( 1 month)
Intestine of the humans、pigs、rats、cats、dogs
Trichinellosis is acquired by ingesting meat containing cysts (encysted larvae) of Trichinella.
After exposure to gastric acid and pepsin, the larvae are released
from the cysts and invade the small bowel mucosa where they develop into adult worms
(life span in the small bowel: 4 weeks).
After 1 week, the females release larvae
that migrate to the striated muscles where they encyst
< Infection occurs by ingesting encysted larvae in undercooked meat.
< Larvae penetrate the intestinal wall and move to muscle tissue where they encyst in individual cells (nurse cells). Active muscles, such as the diaphragm and tongue, often have the greatest numbers of larvae.
< Adults attach to the intestinal mucosa and being to release larvae in one week. The adults live for about 4 weeks and may release more than 1000 larvae.
Pathogenesis and clinical manifestation
The process of pathological change can be divided three phases.
1)Invade phase : intestinal inflammation
For invading of larvae and adult worms, the wall of intestine is damaged
2) Migratory phase : toxic and allergic
severe systemic disease
allergic phenomena such as edema, pneumonitis, and pleural transudate
3) Encystation of the larvae and tissue
repair formation of cyst
clinical manifestation (Symptoms)
Infection with Trichinella spiralis may be asymptomatic, especially in light infections. Adults in the intestine may cause diarrhea, abdominal pain, and vomiting. Larvae moving into the tissues may cause facial swelling, fever, muscle pain, splinter hemorrages (under fingernails) or rashes.
Heavy infections may lead to heart problems or central nervous system involvement. Large numbers of larvae in other muscles may lead to soreness and weakness which often lessens over time.
Trichinella spiralis (T-1) (green) is the most common member of the genus. The world distribution as depicted is almost certainly an under-representation
Prevention and control
avoidance of eating raw or undercooked pork and meat of other wild animals; and avoidance of feeding raw garbage to pigs will prevent transmission of infection to man.