Chapter ten serology
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Chapter Ten - Serology. FRSC 8104 Criminalistics II Professor Bensley. FRSC 8104 – Serology Objectives. Describe the basic components of blood Convey the techniques for collecting and preserving blood evidence at a crime scene.

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Chapter Ten - Serology

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Chapter ten serology

Chapter Ten - Serology

FRSC 8104

Criminalistics II

Professor Bensley


Frsc 8104 serology objectives

FRSC 8104 – Serology Objectives

  • Describe the basic components of blood

  • Convey the techniques for collecting and preserving blood evidence at a crime scene.

  • List and describe various forensic tests used to characterize a stain as blood.

  • Understand the concept of antigen-antibody interaction and how it is applied to the origin determination of blood.


Frsc 8104 serology objectives1

FRSC 8104 – Serology Objectives

  • List the A-B-O antigens and antibodies found in the blood for each of the four blood types: A, B, AB, and O.

  • Understand and describe how whole blood is typed.

  • Describe the structure of components of semen and understand the laboratory tests used to characterize seminal stains.


Frsc 8104 serology outline

FRSC 8104 – Serology Outline

  • Introduction

    • What is serology?

    • Three main goals of Forensic Serology

    • What is proteomics?

  • Blood

    • Formed Elements

      • Erythrocytes (RBC)

      • Leukocytes (WBC)

        • Lymphocytes

        • Monocytes

        • Granulocytes

      • Platelets


Frsc 8104 serology outline1

FRSC 8104 – Serology Outline

  • Fluid (Native Plasma)

    • Serum = Plasma - fibrinogen

http://home.comcast.net/~itpaware/platelets.html


Chapter ten serology

http://www.pennmedicine.org/health_info/bloodless/000209.html


Frsc 8104 serology outline2

FRSC 8104 – Serology Outline

  • Collection of Blood Evidence

  • Factors Affecting Blood Evidence

  • Presumptive Tests for Blood

    • Introduction – Catalytic Tests

    • General Reaction

    • False positives and False negatives

    • Color Tests

      • Kastle-Meyer

      • Tetramethylbenzidine

      • Leucomalachite Green


Frsc 8104 serology outline3

FRSC 8104 – Serology Outline

  • Other Presumptive Tests

    • Chemiluminescent Tests (Luminol)

    • Fluorescent Tests (Fluorescein)

http://www.webpagedreams.com/ABPD/Investigation.htm


Frsc 8104 serology outline4

FRSC 8104 – Serology Outline

  • Confirmatory Tests for Blood

    • Crystal Tests

      • Takayama Test

  • Antigens and Antibodies, Etc.

    • Antigens

      • Definition

      • Types

      • Requirements


  • Frsc 8104 serology outline5

    FRSC 8104 – Serology Outline

    • Antibodies

      • Definition

      • Types

      • Structure

      • Classes

    • Antigen/Antibody Reactions

    • Antisera


    Frsc 8104 serology outline6

    FRSC 8104 – Serology Outline

    • Origin Determination Examinations

      • False Positives

      • False Negatives

      • Ring Precipitin Test

    http://diverge.hunter.cuny.edu/~weigang/Lecture-syllabus.html


    Frsc 8104 serology outline7

    FRSC 8104 – Serology Outline

    • Ouchterlony Double Diffusion


    Chapter ten serology

    http://www.nfstc.org/pdi/Subject02/pdi_s02_m02_02_b.htm


    Frsc 8104 serology outline8

    FRSC 8104 – Serology Outline

    • Blood Group Systems

      • ABO System

        • Introduction

        • Genetics

    http://bloodcenter.stanford.edu/about_blood/blood_types.html


    Frsc 8104 serology outline9

    FRSC 8104 – Serology Outline

    • Body Fluids

      • Seminal Fluid

        • Pipeline Effect

          • Pre-Sperm – Cowper’s Gland

          • Sperm-Rich – Prostate Gland

          • Post-Sperm – Seminal Vasicular Secretion

        • Spermatozoa

          • Morphology

          • Production

          • Their Fate


    Frsc 8104 serology outline10

    FRSC 8104 – Serology Outline

    • Seminal Plasma

    • Prostatic Acid Phosphatase

      • Introduction

      • Testing Procedures

    • Choline

    • Spermine

      • Barberios Test

    • P30 (PSA)


    Frsc 8104 serology outline11

    FRSC 8104 – Serology Outline

    • Saliva

      • Introduction

      • Secretors vs. Non-Secretors

      • Formation

        • Parotid Gland

        • Submandibular (Submaxillary)

          Gland

        • Sublingual Glands

      • Composition

      • Functions

      • Testing

        • Amylase – Phedebas Test


    Frsc 8104 serology outline12

    FRSC 8104 – Serology Outline

    • Secretor System

    • Lewis System

      • Introduction

      • Genetics

    • Urine

      • Composition

      • Properties

      • Testing Procedures


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