Rhogtpases and actin cytoskeleton
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RhoGTPases and Actin Cytoskeleton. Alex André Andres Elena Isabelle Shuoshuo. Structural & Functional Predictions for ELMO homologues A Bioinformatic Approach. Overview. Cytoskeletal dynamics in cellular processes Rho GTPases play role in cytoskeletal remodeling

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RhoGTPases and Actin Cytoskeleton

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RhoGTPases and Actin Cytoskeleton

Alex

André

Andres

Elena

Isabelle

Shuoshuo

Structural & Functional

Predictions for

ELMO homologues

A Bioinformatic Approach


Overview

  • Cytoskeletal dynamics in

    cellular processes

  • Rho GTPases play role in

    cytoskeletal remodeling

  • GEFs and GAPs regulate

    Rho GTPases

  • ELMO1 – GEF for Rac

Actin cytoskeleton


ELMO1

(Engulfment and cell motility protein 1)

  • CED12 in C.elegans

    phagocytosis of apoptotic cells and cell motility

  • Not much known (19 papers in Pubmed)

  • What shall we do?

BIOINFORMATICS


ELMO1 domain organisation


Alignment of the ELMO-domain


Alignment of the PH-domain


Phylogenetic relationship of ELMO1 homologues

plants

yeasts

Nematodes

100

100

vertebrates

vertebrates

vertebrates

vertebrates

vertebrates

64

100

100

100

insects

100


Biocomputing and Reality


Prediction of phosphorylation sites in the ELMO1 sequence

AKT1 Rac-alpha S/T kinase Hck haematopoetic cell kinase


Structure prediction of PH domain by homology remodelling

PH domain identity Geno3D Sequence Identity 23.6%

3 models were predicted by homology modelling


Proper homology modelling can yield significantly more information than conventional analysis


side view

overhead view


The ELMO team


“Critiques help us to progress”


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