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Micropipet. How to use a micropipet.

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Presentation Transcript
how to use a micropipet
How to use a micropipet
  • In science, there are many different ways to measure volume.  One very important tool to a molecular biologist is a micropipet.  A micropipet measures in micro liters ( millionths of liters).  Some of the pipet measure very small volumes and some measure larger volumes.  Each of these pipettes must have a disposable pipet tip on them.
the micropipet
The micropipet

Video on how to Adjust Dial

slide4
How to place a tip on the pipet
  • Take the pipet and place into a tip in the tip box.
how to use the pipet to load a gel
How to Use the Pipet to Load a Gel
  • Press the plunger down to the first soft stop.  You will feel it stop naturally
lower the tip into the loading dye and slowly let the plunger come back up and fill the pipet
Lower the tip into the loading dye and slowly let the plunger come back up and fill the pipet.
slide10

Press down to the first soft stop to deliver the loading dye.  If it all doesn\'t come out you can push down to the hard stop to give it one last chance to come out.

important
Important!!!!!!
  • Important! Don\'t lift up on the plunger until after you have pulled the tip out of the sample tube.
slide12

How to Eject a Tip Once you have Used itTip is ejected by placing finger on Tip Ejector ButtonPress Down on Ejector Button Holding the pipet over a waste cup

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