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Micropipet. How to use a micropipet.

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How to use a micropipet
How to use a micropipet

  • In science, there are many different ways to measure volume.  One very important tool to a molecular biologist is a micropipet.  A micropipet measures in micro liters ( millionths of liters).  Some of the pipet measure very small volumes and some measure larger volumes.  Each of these pipettes must have a disposable pipet tip on them.


The micropipet
The micropipet

Video on how to Adjust Dial




Yeah good job
Yeah! Good Job. seat the tip


How to use the pipet to load a gel
How to Use the Pipet to Load a Gel seat the tip

  • Press the plunger down to the first soft stop.  You will feel it stop naturally


Lower the tip into the loading dye and slowly let the plunger come back up and fill the pipet
Lower the tip into the loading dye and slowly let the plunger come back up and fill the pipet.



Press down to the first soft stop to deliver the loading dye.  If it all doesn't come out you can push down to the hard stop to give it one last chance to come out.


Important
Important!!!!!! dye.  If it all doesn't come out you can push down to the hard stop to give it one last chance to come out

  • Important! Don't lift up on the plunger until after you have pulled the tip out of the sample tube.


How to Eject a Tip Once you have Used it dye.  If it all doesn't come out you can push down to the hard stop to give it one last chance to come outTip is ejected by placing finger on Tip Ejector ButtonPress Down on Ejector Button Holding the pipet over a waste cup


  • Video on how to eject a tip dye.  If it all doesn't come out you can push down to the hard stop to give it one last chance to come out


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