Harvesting Energy

1. Harvesting Energy • Glycolysis and Cellular Respiration

2. Energy from Glucose • __________and _______________ break glucose and other carbon compounds apart. • Energy released from broken bonds is harnessed to make ____ to run cell processes. • ALL Eukaryotic organisms (including plants) carry out cellular respiration ____ _____ _____.

3. Cellular respiration takes place in the mitochondria. mitochondrion outer membrane intermembrane space inner membrane matrix cristae

4. Remember ATP? Adenosine triphosphate, the universal energy carrier, is a single nucleotide (adenine) with two extra phosphate groups attached. ATP is produced in great quantities by mitochondria.

5. Review: ATP is produced and used in coupled reactions ______________ (glucose breakdown) _____________ (ATP synthesis) ______________ (ATP breakdown) _______________ (protein synthesis) ADP + heat net ____________ “downhill” reaction

6. Overview ____________ splits sugar into two 3-carbon chains (pyruvate), producing 2 ATPs glucose 2 ATP 2 NADH glycolysis (cytosol) 2 pyruvate 2 NADH 2 acetyl CoA ________________ breaks the sugar down further, producing 32-34 ATPs. Krebs (citric acid) cycle 6 NADH 2 ATP (mitochondrion) 2 FADH2 32 or 34 ATPs electron transport chain Total 36 or 38 ATPs NADH and FADH (derived from vitamins B3 and B2) act as electron carriers.

7. in cytosol– no oxygen required glucose if no O2 available fermentation in mitochondria– oxygen required cellular respiration

8. Glycolysis in cytosol ADP ATP ATP ADP 4 4 2 2 2 2 G3P pyruvate glucose fructose bisphosphate NAD+ NADH 2 2 1 Glucose activation 2 Energy harvest __________ is split in half. Net gain of 2 ATPs End product is ________ 2 ATPs are used.

9. Glycolysis Animation Role of B Vitamins

10. Cellular respiration begins with moving the products of glycolysis into the mitochondrion. 1 Formation of acetyl CoA 3 _______ 3 NAD+ FAD _______ ____ coenzyme A coenzyme A CoA 2 Krebs cycle 2 ______ __________ ________ NAD+ _______ ADP In cytosol _______ In mitochondrion

11. Krebs Cycle Animation

12. Electron carriers from the Krebs cycle are used to power an electron transport chain and proton pump. Oxygen is required to accept energy- depleted electrons. Flow of H+ down concentration gradient powers ATP synthesis. High-energy electron carriers from acetyl CoA formation, Krebs cycle, and glycolysis feed into the ETC. H2O 2e– FADH2 2H+ 1/2O2 NADH ATP ADP + Pi FAD matrix NAD inner membrane intermembrane space Energy from high-energy electrons powers active transport of H+ by ETC. High H+ concentration is generated by active transport. H+ channel is coupled to ATP synthesizing enzyme.

13. Electron Transport Chain Animation

14. Main points of cellular respiration • Cellular respiration takes place in the mitochondria. • The product of glycolysis is broken down completely, releasing CO2 as a waste gas. • The electron transport chain/proton pump complex uses energized electrons to produce up to 36 ATPs from each molecule of glucose.

15. Mitochondria animation on XVIVO

16. glucose (cytosol) In the absence of _________, cellular respiration cannot continue. Pyruvate from glycolysis is fermented, producing ____ ____ or ______. 2 NADH 2 ATP glycolysis lactate Cellular respiration requires ______ to pick up electrons and protons at the end of an electron transport chain or 2 pyruvate fermentation (no O2) ethanol + CO2 cellular respiration 2 CO2 2 NADH 2 acetyl CoA 4 CO2 Krebs (citric acid) cycle 2 ATP H2O 1/2O2 6 NADH 2H+ 2 FADH2 2e– 32 or 34 ATPs electron transport chain (mitochondrion)

17. If the mitochondria can’t accept pyruvate, NADH from glycolysis accumulates, depleting NAD+. Fermentation uses excess NADH to convert pyruvate to lactic acid or ethanol. regeneration NAD+ NADH NADH NAD+ NADH 2 2 (glycolysis) (fermentation) glucose pyruvate lactate 2 ATP ADP 2

18. Fatty acids have more energy per gram than sugars, so are preferred as energy storage. Other monomers can be broken down for energy as well. (cytosol) complex carbohydrates fats proteins glucose amino acids glycerol Glycolysis fatty acids pyruvate acetyl CoA While brain cells usually must have glucose, other cells can use fatty acids and amino acids for energy. Amino acids must be stripped of nitrogen first, which is disposed of in the kidneys. Krebs cycle electron carriers Electron transport chain synthesis breakdown (mitochondrion)

19. What if you take in more monomers than you need to produce ATP? Simple sugars are broken apart in glycolysis. If the mitochondria does not need Acetyl CoA, the acetate is released and can be used to synthesize fatty acids to make fats for storage.

20. Amino acids not needed for protein synthesis are stripped of their amine groups. The remaining carbon backbone can either be used to make Acetyl CoA, or can be used to synthesize fatty acids.

21. Recap • Cellular respiration breaks organic compounds down into inorganic compounds. • In the process, chemical energy in compounds such as sugar is converted to chemical energy in ATP. • ATP is used to run cellular processes. • ALL Eukaryotic organisms carry out cellular respiration.