Supplementary Figures:
This presentation is the property of its rightful owner.
Sponsored Links
1 / 7

Supplementary Figures: PowerPoint PPT Presentation


  • 45 Views
  • Uploaded on
  • Presentation posted in: General

Supplementary Figures: Fig. 1, 2, and 3 show H1299 cells overexpressing Set7/9wt or H297A catalytic mutant and U2-OS, U2-OS Set7/9KD, and U2-OS Set7/9 KD cells expressing ectopic Set7/9wt or its H297A mutant. These cell lines were treated with different sources of DNA

Download Presentation

Supplementary Figures:

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Supplementary figures

Supplementary Figures:

Fig. 1, 2, and 3 show H1299 cells overexpressing Set7/9wt or H297A catalytic mutant and U2-OS, U2-OS Set7/9KD, and

U2-OS Set7/9 KD cells expressing ectopic Set7/9wt or its H297A mutant. These cell lines were treated with different sources of DNA

damage: doxorubicin (1), gamma- (2), or UV-irradiation (3). Following the treatment with genotoxic stress cells were harvested at different

time points, stained with Propidium Iodine and analysed by FACS for their cell cycle distribution. In all the cases the lack of Set7/9 resulted in

a more pronounced fraction of cells arrested in G1/S phase. Addition of the ectopically expressed Set7/9wt or H297A mutant into shRNA-

Set7/9 cells resulted in different effects: Set7/9wt, but not Set7/9mut, partially restored normal DDR (DNA Damage Response) of U2-OS cells.

Overexpression of Set7/9mut in H1299 cells (p53- ) despite the presence of the endogenous Set7/9 protein, also resulted in significant

G1/S arrest. Collectively, these data suggest that Set7/9 is important for cell cycle progression in response to various forms of DNA Damage (DD).

Fig. 4. The bioinformatics analysis demonstrates that the positive correlation between E2F1 and TP73 expression positively affects survival of

the lung cancer patients (see description of the experiment provided for Fig. 5). Left panel shows that low expression of E2F1 (green line) in lung cancer patients correlates with better survival. The two other upper panels denote groups with strong positive correlation (middle) between E2F1 and TP73 expression and negative correlation (right). The lower panel shows the rate of survival for patients with positive (green line) and negative (red line) correlations.

Fig. 5. A breast cancer data set shows that the negative correlation between Set7/9 and TP73 is associated with a better prognosis for survival. Left panel provides statistically significant correlation (p=0.015) between low expression of E2F1 and better survival (see description of the experiment provided for Fig. 5).

Table 1. A summary of Set7/9, Cyclin E, E2F1, and TP53 expression levels in six different lung cancer cell lines and their cell cycle and apoptotic

response to DNA Damage induced by doxorubicin.


Supplementary figures

H1299 cells (p53-)

U2-OS cells (p53+)

no treatment

no treatment

14 hrs doxorubicin

24 hrs doxorubicin

24 hrs doxorubicin

control

Set7/9KD+Set7/9mut

Set7/9KD+

Set7/9wt

control

Set7/9wt

Set7/9mut

Supplementary Fig.1


Supplementary figures

U2-OS-Set7/9 KD

U2-OS control

0 hrs X-rays

16 hrs X-rays

48 hrs X-rays

Supplementary Fig.2


Supplementary figures

U2-OS control

Set7/9KD (LSL)

0 hrs

UV

8 hrs

UV

16 hrs

UV

Supplementary Fig.3


Supplementary figures

Positive correlation between  E2F1  and  TP73 expression increases survival in lung cancer, where E2F1 is an oncogene

 E2F1 p= 0.016

Group=1: green , strong positive correlation (0.53) between E2F1 and TP73

Group=2: red , no (or negative) correlation (-0.53) between E2F1 and TP73

GEO dataset ID: GSE36471

GEO dataset Type: lung cancer

p= 0.03

GEO dataset ID: GSE36471

GEO dataset Type: lung cancer

Supplementary Fig.4


Supplementary figures

Inverse correlation between SETD7 and TP73 expression in breast cancer, where E2F1 is an oncogene, increases survival

 E2F1 p= 0.015

Group=1: green , strong positive correlation (0.73) between SETD7 and TP73

Group=2: red , no (or negative) correlation (-0.33) between SETD7 and TP73

GEO dataset ID: GSE21653

GEO dataset Type: Breast Cancer

 p= 0.025

GEO dataset ID: GSE21653

GEO dataset Type: Breast Cancer

Supplementary Fig.5


Supplementary figures

Supplementary Data: Table 1

name

p53wt


  • Login