Green fluorescent protein l.jpg
This presentation is the property of its rightful owner.
Sponsored Links
1 / 13

Green Fluorescent Protein PowerPoint PPT Presentation


  • 78 Views
  • Uploaded on
  • Presentation posted in: General

Green Fluorescent Protein. a B/MB senior seminar brought to you by Colm O’Carroll. This presentation will cover . The structural aspects of GFP which make fluorescence possible The advantages of using GFP and GFP mutants over other fluorescent markers

Download Presentation

Green Fluorescent Protein

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Green fluorescent protein l.jpg

Green Fluorescent Protein

a B/MB senior seminar

brought to you by Colm O’Carroll


This presentation will cover l.jpg

This presentation will cover

  • The structural aspects of GFP which make fluorescence possible

  • The advantages of using GFP and GFP mutants over other fluorescent markers

  • The use of GFP to monitor viral movement in plants


The green fluorescent protein l.jpg

The Green Fluorescent Protein


Gfp s unique structure l.jpg

GFP’s unique structure

  • Composed of 238 amino acids

  • “Paint in a can”

  • Each monomer composed of a central -helix surrounded by an eleven stranded cylinder of anti-parallel -sheets

  • Cylinder has a diameter of about 30A and is about 40A long

  • Fluorophore located on central helix


The active site l.jpg

The Active Site


The fluoropore active site l.jpg

The Fluoropore Active Site

  • Ser65-Tyr66-Gly67

  • Deprotonated phenolate of Tyr66 is cause of fluorescence

  • Forster Cycle (1949-Theodor Forster)

  • Proton transfer to His148


Fluorophore formation l.jpg

Fluorophore formation

  • One limitation of wtGFP is its slow rate of fluorescence acquisition in vivo

  • Renaturation most likely by a parallel pathway

  • Oxidation of Fluoropore (2-4 hours)

  • Two step process


  • Login