Thiol quantification. Professor ： Chau , Lai-Kwan Student ： Liu, Wan-Chun Date ： 2013/11/08. Method. 2% MPDMS, or 2% MPTMS. plasma. 0 .004mM DTNB. Glass slide. UV. 12hr. 15min. 16hr. Optimization - concentration of DTNB. Mechanism of E llman’s reaction.
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Strong reductant : DTT
Weak reductant: 2-mercaptoethanol
Thiol coverage on glass surface