Thiol quantification
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Thiol quantification. Professor : Chau , Lai-Kwan Student : Liu, Wan-Chun Date : 2013/11/08. Method. 2% MPDMS, or 2% MPTMS. plasma. 0 .004mM DTNB. Glass slide. UV. 12hr. 15min. 16hr. Optimization - concentration of DTNB. Mechanism of E llman’s reaction.

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Thiol quantification

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Thiol quantification

Thiol quantification

Professor:Chau, Lai-Kwan

Student:Liu, Wan-Chun

Date:2013/11/08


Thiol quantification

Method

2%MPDMS, or

2% MPTMS

plasma

0.004mMDTNB

Glassslide

UV

12hr

15min

16hr


Optimization concentration of dtnb

Optimization - concentration of DTNB


Mechanism of e llman s reaction

Mechanism of Ellman’s reaction


Verify by strong weak reducing agents

Verify by strong/weak reducing agents

Strong reductant : DTT

Weak reductant: 2-mercaptoethanol


Dtnb reacts with mptms mpdms

DTNB reacts with MPTMS/MPDMS


Standard curve

Standard curve

(r2=0.998)


Concentration of thiols on glass surface

Concentration of thiols on glass surface


Thiol c overage on glass surface

Thiolcoverage on glass surface

5 cm

(SH/nm2)

0.2 cm

0.8 cm


Thiol quantification

Thiol coverage on glass surface

0.7 nm

0.4nm


Future work

Future work

  • Gold coverage on MPDMS/MPTMS modified glass surface.


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