Immunological characteristics of patients infected with common intestinal helminths : result of a st...
Download
1 / 32

Source:Nongkhai Provincial office - PowerPoint PPT Presentation


  • 69 Views
  • Uploaded on

Immunological characteristics of patients infected with common intestinal helminths : result of a study based on reverse-transcriptase PCR S.Lertanekawattana,T.Wichatrong,K.Chaisari, R.Uchikawa and N.Arizono

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Source:Nongkhai Provincial office' - rangle


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript

Immunological characteristics of patients infected with common intestinal helminths : result of a study based on reverse-transcriptase PCR

S.Lertanekawattana,T.Wichatrong,K.Chaisari, R.Uchikawa and N.Arizono

Nongkhai Hospital and Department of Medical Zoology,

KyotoPrefectural University of Medicine


Source:Nongkhai Provincial office common intestinal helminths : result of a study based on reverse-transcriptase PCR


Long-term infection intestinal parasites : common intestinal helminths : result of a study based on reverse-transcriptase PCR

-Malnutrition

-Retardation of the intelectual

-Impair physical growth of children

Oberhelman et al.,1998:


Helminth infection : common intestinal helminths : result of a study based on reverse-transcriptase PCR

-Impair immune response to virus,bacteria protozoa and even oral vaccine

( Cooper et al, 2001)


Ascaris and Hook worm induce expansion of the Th2 lymphocyte subset and regulate IgE antibody production, eosinophilia and mastocytosis

(Pritchard et al,1995)


Th2 cytokines have antagonistic effects on the expansion of the Th1 lymphocyte subset

(Cooper et al,2001)


Anisakiasis the Th1 lymphocyte subset

Clinical : Urticaria,Angioedema,

Anaphylaxis

Immunological response :

IgE , mast cell, basophil

Th 2 interleukin 4,predominant


Villus pathology induced by the rodent nematode the Th1 lymphocyte subsetNippostrongylus brasiliensis

  • Nippostrongylus brasiliensis


4 the Th1 lymphocyte subset

3

2

1

E-cadherin expression decreases at villus tips after infection

Day 0

Day 10

E-cadherin score

0

28

7

10

14

days after infection

E-cadherin immunohistochemistry


Pathological changes develop in the intestinal mucosa,including partial villus atrophy and crypt hyperplasia,decreasing of sucrase and alkaline phosphatase activity,deterioration of epithelial permeability and barrier function

(Hyoh et al,2002 ; Sekikawa et al;2003)


Glucose transport in the small intestine mucosa,including partial villus atrophy and crypt hyperplasia,decreasing of sucrase and alkaline phosphatase activity,deterioration of epithelial permeability and barrier function

Lumen

SGLT1 (Na-driven glucose cotransporter)

glucose

Na

*

*

Absorptive

cell

glucose

Na

K

Na-K ATPase

Lamina propria

glucose

Na


Infection with intestinal nematodes in rodents causes induction Th2 cytokines such as IL-4.IL-5and IL-13 and induce IgE antibody responses, eosinophilia and intestinal mastocytosis

(Matsuda et al, 1995 ; Garside et al,2000)


Aim of study induction Th2 cytokines such as IL-4.IL-5and IL-13 and induce IgE antibody responses, eosinophilia and intestinal mastocytosis :

To determine whether common helminth infections could modify the intestinal immunopathological status of the host,by the expression in the human duodenal mucosa of cytokines,eosinophil,mast cell specific molecule,monosaccharide transporters of the glucose-transporter (GLUT)


Subjects : induction Th2 cytokines such as IL-4.IL-5and IL-13 and induce IgE antibody responses, eosinophilia and intestinal mastocytosis

31 patients with gastrointestinal problems and indicated for gastroduodenal fiberscopy as diagnotic measurement and did not have dysenteric disease and gave their written,informed consent for the study


Materials: induction Th2 cytokines such as IL-4.IL-5and IL-13 and induce IgE antibody responses, eosinophilia and intestinal mastocytosis

Duodenal biopsy specimens


Methods : induction Th2 cytokines such as IL-4.IL-5and IL-13 and induce IgE antibody responses, eosinophilia and intestinal mastocytosis

Semi-quantitative RT-PCR for IL-5 ,IL-13, IFN- , GLUT-1 and GLUT-5


Laboratory study: induction Th2 cytokines such as IL-4.IL-5and IL-13 and induce IgE antibody responses, eosinophilia and intestinal mastocytosis

At central lab. of Kyoto Prefectural University of Medicine


Density analysis of PCR products induction Th2 cytokines such as IL-4.IL-5and IL-13 and induce IgE antibody responses, eosinophilia and intestinal mastocytosis

Electrophoresis on agar each band was evaluated using NIH Image software, the band densities were normalized relative to those of ß-actin


Result induction Th2 cytokines such as IL-4.IL-5and IL-13 and induce IgE antibody responses, eosinophilia and intestinal mastocytosis


Figure 1 induction Th2 cytokines such as IL-4.IL-5and IL-13 and induce IgE antibody responses, eosinophilia and intestinal mastocytosis Some of PCR results,showing the expression of IL-13,IL-5,IFN-,andfor reference,-actin in the duodenal mucosa of the 31 patients


Figure 2 (a) The frequencies of expression of IL-5( )an induction Th2 cytokines such as IL-4.IL-5and IL-13 and induce IgE antibody responses, eosinophilia and intestinal mastocytosisd IL-13 ( )

In the duodenal mucosa of the 10 patients with eosinophilia and the 21 without


Figure 3 (b) In the 9 patients with helminth infection(on presentation or in the previous 3 months ) and the 22 without

P=0.041


Summary: presentation or in the previous 3 months ) and the 22 without

The present findings indicate that infections with common intestinal helminths, including Taeniasis are likely to modify mucosal immunology so that it shifts to a Th2-predominant status.

SGUT-1 and GLUT-5 no significant decrease in patients with helminth infection


Acknowledgment: presentation or in the previous 3 months ) and the 22 without

This work was supported by Grants-in-Aid for Research from the Japan Society for the Promotion of Science and the Japanese Ministry of Health,Labour and Welfare


ad