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P2 HRI Wellesbourne Brian Thomas Lol Trueman Brian Smith P3 University of Liverpool

GARLIC & HEALTH. P2 HRI Wellesbourne Brian Thomas Lol Trueman Brian Smith P3 University of Liverpool Hamish Collin Meriel Jones Brian Tomsett. WP4 Garlic Sulphur Biochemistry. GARLIC & HEALTH.

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P2 HRI Wellesbourne Brian Thomas Lol Trueman Brian Smith P3 University of Liverpool

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  1. GARLIC & HEALTH • P2 HRI Wellesbourne • Brian Thomas • Lol Trueman • Brian Smith • P3 University of Liverpool • Hamish Collin • Meriel Jones • Brian Tomsett WP4 Garlic Sulphur Biochemistry

  2. GARLIC & HEALTH • To establish the limiting biochemical and physiological steps for allicin formation in garlic • Biochemical pathway • Integration with development • Genes for limiting steps Garlic Sulphur Biochemistry

  3. GARLIC & HEALTH Pathway Biochemistry

  4. GARLIC & HEALTH Pathway Biochemistry (II) CSO3 Alliinase Tissue damage Pyruvate Ammonia S alk(en)yl sulfenic acids Organosulphur compounds Allicin

  5. GARLIC & HEALTH Integration with development

  6. GARLIC & HEALTH • Radiolabelled intermediates identified using HPLC and HPLC-MS in comparison with chemically synthesised compounds (P3). • Differential display sequences and isolated and reanalysed by northern blots and in situ hybridisation, where possible, with other Allium sp. (P3) • First year harvests and analysis completed (P2). • Second year experimental material planted. (P2). • cDNA clones encoding alliinase isolated and sequenced (P2). Second Year Milestones

  7. GARLIC & HEALTH • DP.16: Pathway intermediates identified (P2, P3) • DP.17: First sulphur budget for garlic (P2) • DP. 18: Clones for alliinase Second Year Deliverables

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