Introduction to Microbiology and Laboratory Safety. Biosafety. Introduction to Microbiology and Lab Safety. Use and Care of Microorganisms
Use and Care of Microorganisms
This area of science, may involve many dangers and hazards while experimenting. It is the sole responsibility of all teacher(s)/sponsor(s) to teach students proper safety methods and sterile techniques.
Roles and Responsibilities of Students & Adults
The Instructor is responsible for working with the student to evaluate any possible risks involved in order to ensure the health and safety of the student conducting the research and the humans or animals involved in the study.
General Lab Use - Hypochlorite Solutions
The use of a physical or chemical procedure to virtually eliminate all recognized pathogenic microorganisms but not all microbial forms (bacterial endospores) on inanimate objects.
Sterilization: The use of physical or chemical procedures that destroy all microbial life forms, including highly resistant bacterial endospores.
Autoclave: Pressurized steam at 15 psi and 121oC for an average of 20 min (10 – 40 min depending on bulk and load)
Required for all microbiology preparations to assure that contaminants are not introduced.
The inoculating loop is usually used for making transfers of bacterial cultures (see next few slides for technique).
Label top side of slide with small piece of masking tape (or anything that won’t wash off)
Prepare slide and air dry (circle specimen on bottom of slide with sharpie to enable finding specimen when you put it on the scope)
Diagnostic bacteriology is concerned with the isolation and identification of bacteria in a specimen from a patient.
Microbes placed in 4 categories:
Biosafety Levels (BSL 1-4)