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Vector NTI ( Invitrogen ). 源資國際生物科技公司 業務專員 陳良坤. Vector NTI Suite.  Vector NTI Nucleotide analyses Restriction site analysis Design Primers  Align  Assemble. Create a new folder. Create a new sequence. View a sequence. Main Menu. Main Toolbar. Pane Selection Toolbar.

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vector nti invitrogen

Vector NTI (Invitrogen)

源資國際生物科技公司 業務專員 陳良坤

slide2

Vector NTI Suite

  • Vector NTI
  • Nucleotide analyses
  • Restriction site analysis
  • Design Primers
  •  Align
  •  Assemble
slide7

Main Menu

Main Toolbar

Pane Selection Toolbar

Pane Toolbar

Text Pane

Graphics Pane

Sequence Pane

reverse sequenece
Reverse sequenece

Select all sequence

→Right-click mouse

→Reverse selection

slide14

Translation

Select ORF

→Analyses

→Into New Protein

→Direct Strand

restriction site analyses
Restriction site Analyses

→Analyses

→Restriction Analyses

→Restriction Sites

cloning
Cloning

by PstI

design primers
Design Primers

Select region

→Analyses

→Primer Design

→Find PCR Primers

slide21

Dimmer and Hairpin of Primers

Select primer

→Right-click mouse

→ Analyses

alignment
Alignment

Select file

Align

→AlignX-Align Selected Molecules

assemble
Assemble

Assemble

→ContigExpress-Assemble Selected Sequence

slide26

Assemble with ABI files

Open ContigExpress Project

→Project

→Add Fragments

→From ABI file (no Chinese)

export data
Export data

→ Right-click mouse

→Camera

→Select sequence

→ Right-click mouse

→Copy sequence…..

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