Are there microbes lurking
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June 12, 2012 | State University of NY. Paul W. Tranchell CIH, CSP, RBP Project Manager. Are there Microbes Lurking?. June 12, 2012 | State University of NY. Are there Microbes Lurking?. Industrial Hygiene. Occupational Stressors: (i.e. biological agents). Anticipation Recognition

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Are there Microbes Lurking?

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Are there microbes lurking

June 12, 2012 | State University of NY

Paul W. Tranchell CIH, CSP, RBP

Project Manager

Are there Microbes Lurking?

Are there microbes lurking1

June 12, 2012 | State University of NY

Are there Microbes Lurking?

Occupational stressors i e biological agents

Industrial Hygiene

Occupational Stressors: (i.e. biological agents)

  • Anticipation

  • Recognition

  • Evaluation

  • Control




Normal Locations

Selected Suppliers

Unusual & Ancillary Biohazards

Biosafety Fundamentals



  • The application of knowledge, techniques and equipment to prevent personal, laboratory, and environmental exposure to potentially infectious agents or biohazards

  • Biosafety defines the containment conditions under which infectious agents can be safely manipulated

  • The objective of containment is to confine biohazards and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents


Normal locations

Normal Locations

  • Bloodborne Pathogens

  • Microbiological Laboratories

  • Animal Laboratories

  • Molecular Biology Labs

Select suppliers

Select Suppliers

  • American Type Culture Collection (ATCC) - Repository of a huge number of cell lines

  • CABRI (Common Access to Biological Resources and Information)

  • Developmental Studies Hybridoma Bank - Monoclonal antibodies

  • DSMZ - The DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH = German Collection of Microorganisms and Cell Cultures) is a non-profit public domain general repository specializing in tumor cell lines. It holds the largest collection of human hematopoietic cell lines currently available worldwide.

  • European Collection of Cell Cultures (ECACC) - ECACC "was established in 1984 as a cell culture collection to service the research community and provide an International Depository Authority recognised patent depository for Europe."

  • HyperCLDB - Hypertext resource derived from the Cell Line Data Base (CLDB) with descriptions of more than 4,000 cell lines

  • National Cell Culture Center - A "national resource facility established by the National Institutes of Health (NIH) to provide customized, large scale, cell culture services for basic research laboratories."

Unusual ancillary hazards

Unusual & Ancillary Hazards

  • Field Work

    • Lyme Disease

    • Insect Bites

    • Suborder Surpentes

    • Rodent Diseases

      • Plague

      • Hanta Virus

  • HVAC / Legionella

  • Bird Droppings (no bleach please)

  • Mold

  • Suspicious Packages / ”white” powder

  • Drum Skins

Chain of infection

Biosafety Fundamentals

Chain of Infection

Risk Assessment

Reservoir of pathogen





Route of entry / infectious dose

Susceptible host

Incubation period


Practices / Equipment

Access control / Immunization




Biosafety Fundamentals



Control strategies

Biosafety Fundamentals

Control Strategies

  • Standard Microbiological Practices

  • Special Microbiological Practices

  • Safety Equipment

    • Primary Barrier

      • Biological Safety Cabinets (BSC)

      • Types, Selection, Use!

    • Personal Protective Equipment

  • Laboratory Facilities

    • Secondary Barriers


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