Are there microbes lurking
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June 12, 2012 | State University of NY. Paul W. Tranchell CIH, CSP, RBP Project Manager. Are there Microbes Lurking?. June 12, 2012 | State University of NY. Are there Microbes Lurking?. Industrial Hygiene. Occupational Stressors: (i.e. biological agents). Anticipation Recognition

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Are there Microbes Lurking?

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Are there microbes lurking

June 12, 2012 | State University of NY

Paul W. Tranchell CIH, CSP, RBP

Project Manager

Are there Microbes Lurking?


Are there microbes lurking1

June 12, 2012 | State University of NY

Are there Microbes Lurking?


Occupational stressors i e biological agents

Industrial Hygiene

Occupational Stressors: (i.e. biological agents)

  • Anticipation

  • Recognition

  • Evaluation

  • Control


Agenda

Agenda

Definition

Normal Locations

Selected Suppliers

Unusual & Ancillary Biohazards

Biosafety Fundamentals


Biosafety

Biosafety

  • The application of knowledge, techniques and equipment to prevent personal, laboratory, and environmental exposure to potentially infectious agents or biohazards

  • Biosafety defines the containment conditions under which infectious agents can be safely manipulated

  • The objective of containment is to confine biohazards and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents

  • Medicine.net http://www.medterms.com/script/main/art.asp?articlekey=33817


Normal locations

Normal Locations

  • Bloodborne Pathogens

  • Microbiological Laboratories

  • Animal Laboratories

  • Molecular Biology Labs


Select suppliers

Select Suppliers

  • American Type Culture Collection (ATCC) - Repository of a huge number of cell lines

  • CABRI (Common Access to Biological Resources and Information)

  • Developmental Studies Hybridoma Bank - Monoclonal antibodies

  • DSMZ - The DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH = German Collection of Microorganisms and Cell Cultures) is a non-profit public domain general repository specializing in tumor cell lines. It holds the largest collection of human hematopoietic cell lines currently available worldwide.

  • European Collection of Cell Cultures (ECACC) - ECACC "was established in 1984 as a cell culture collection to service the research community and provide an International Depository Authority recognised patent depository for Europe."

  • HyperCLDB - Hypertext resource derived from the Cell Line Data Base (CLDB) with descriptions of more than 4,000 cell lines

  • National Cell Culture Center - A "national resource facility established by the National Institutes of Health (NIH) to provide customized, large scale, cell culture services for basic research laboratories."


Unusual ancillary hazards

Unusual & Ancillary Hazards

  • Field Work

    • Lyme Disease

    • Insect Bites

    • Suborder Surpentes

    • Rodent Diseases

      • Plague

      • Hanta Virus

  • HVAC / Legionella

  • Bird Droppings (no bleach please)

  • Mold

  • Suspicious Packages / ”white” powder

  • Drum Skins


Chain of infection

Biosafety Fundamentals

Chain of Infection

Risk Assessment

Reservoir of pathogen

Portal

of

escape

Transmission

Route of entry / infectious dose

Susceptible host

Incubation period

Facilities

Practices / Equipment

Access control / Immunization

PPE

Surveillance


Definitions

Biosafety Fundamentals

Definitions

kcd11/HE.prn/NYCER-Biosafety/NYCER_BSL2&3-121311.pptx


Control strategies

Biosafety Fundamentals

Control Strategies

  • Standard Microbiological Practices

  • Special Microbiological Practices

  • Safety Equipment

    • Primary Barrier

      • Biological Safety Cabinets (BSC)

      • Types, Selection, Use!

    • Personal Protective Equipment

  • Laboratory Facilities

    • Secondary Barriers

kcd11/HE.prn/NYCER-Biosafety/NYCER_BSL2&3-121311.pptx


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