Extraction and Purification. proteins. Selection of Tissue. Choice is based on the type of study Usually select tissue that has large amounts of the materials necessary for the study. Tissues are acquired in a fashion that provides for protection of the system to be studied.
Extraction and Purification
Analytical: used to determine the size of the molecule being studied
Preparative: More common type. Has different type of motors
Fixed angle: most common with fixed angle. The sample tube is in a fixed angle. The force (x g) are reported for the average distance.
Swinging bucket The buckets are able to swing up in to a position such that the sample goes directly down the length of the tube. This allows simple gradient. Samples are at the bottom of the tube rather than on the side.
Precipitation of proteins
Salting out and IEP