Nanobiosensors l.jpg
This presentation is the property of its rightful owner.
Sponsored Links
1 / 20

Nanobiosensors PowerPoint PPT Presentation


  • 141 Views
  • Uploaded on
  • Presentation posted in: General

Nanobiosensors. Sara Huefner November 6, 2006. Outline. Biosensor Background What is a Biosensor? Components of a Biosensor Principles of Detection Biosensors on the Nanoscale Current Research Potential Applications Conclusion. What is a Biosensor?.

Download Presentation

Nanobiosensors

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Nanobiosensors l.jpg

Nanobiosensors

Sara Huefner

November 6, 2006


Outline l.jpg

Outline

  • Biosensor Background

    • What is a Biosensor?

    • Components of a Biosensor

    • Principles of Detection

  • Biosensors on the Nanoscale

    • Current Research

    • Potential Applications

  • Conclusion


What is a biosensor l.jpg

What is a Biosensor?

“Biosensor” once refered to any device which responds to chemical species in biological samples or using biological components.

http://www.gentronix.co.uk/images/newscientist.jpg

www.wvminesafety.org


Current definition for biosensors l.jpg

Current Definition for Biosensors:

A sensor that integrates a biological element with a physiochemical transducer to produce an electronic signal proportional to a single analyte which is then conveyed to a detector.

www.imec.be/ovinter/static_research/BioHome.shtml


Components of a biosensor l.jpg

Components of a Biosensor

Detector

http://www.dddmag.com/images/0409/HTS1_lrg.jpg


1 st component biological element l.jpg

1ST Component: Biological Element

The component used to bind the target molecule.

Must be highly specific, stable under storage conditions, and immobilized.

Microorganism

Tissue

Cell

Organelle

Nucleic Acid

Enzyme

Enzyme Component

Receptor

Antibody

http://www.chemistry.wustl.edu/~edudev/LabTutorials/HIV/DrugStrategies.html


2 nd component physiochemical transducer l.jpg

2ND Component: Physiochemical Transducer

Acts as an interface, measuring the physical change that occurs with the reaction at the bioreceptor then transforming that energy into measurable electrical output.


3 rd component detector l.jpg

3RD Component: Detector

Signals from the transducer are passed to a microprocessor where they are amplified and analyzed.

The data is then converted to concentration units and transferred to a display or/and data storage device.

www.modernmike.com


Principles of detection l.jpg

Principles of Detection

Piezo-Electric

  • measures change inmass

  • measures change in electric distribution

  • measures change in light intensity

  • measures change inheat

Electrochemical

Optical

Calorimetric


Principles of detection10 l.jpg

Principles of Detection

Some piezo-electric devices utilize crystals, such as quartz, which vibrate under the influence of an electric field. The frequency of this oscillation depends on their thickness and cut.

Piezo-Electric Biosensors

Others use gold to detect the specific angle at which electron waves (surface plasmons) are emitted when the substance is exposed to laser light.

electronics.howstuffworks.com

The change in frequency is proportional to the mass of absorbed material.


Principles of detection11 l.jpg

Principles of Detection

  • Amperometric for applied current: Movement of e- in redox reactions detected when a potential is applied between two electrodes.

  • Potentiometric for voltage: Change in distribution of charge is detected using ion-selective electrodes, such as pH-meters.

  • Conductimetric for impedance

Electrochemical Biosensors

http://www.lsbu.ac.uk/biology/enztech/index.html


Principles of detection12 l.jpg

Principles of Detection

  • Colorimetric for color: Measure change in light adsorption as reactants are converted to products.

  • Photometric for light intensity: Photon output for a luminescent or fluorescent process can be detected with photomultiplier tubes or photodiode systems.

Optical Biosensors

www.manimo.it/Prodotti/


Principles of detection13 l.jpg

Principles of Detection

  • If the enzyme catalyzed reaction is exothermic, two thermistors may be used to measure the difference in resistance between reactant and product and, hence, the analyte concentration.

Calorimetric Biosensors

www4.tsl.uu.se/~Atlas/DCS/DCSIL/therm.html


Biosensors on the nanoscale l.jpg

Biosensors on the Nanoscale

Dr. Michael Strano at the University of] Illinois, "We have developed molecular sheaths around the nanotube that respond to a particular chemical and modulate the nanotube's optical properties."

Current Research

http://www.worldchanging.com/archives/001727.html


Biosensors on the nanoscale15 l.jpg

Biosensors on the Nanoscale

SPOT-NOSED Project: A layer of olfactory proteins on a nanoelectrode could react with low-concentration odorants. This technology could be used by doctors to diagnose diseases at earlier stages.

Current Research

http://www.sciencedaily.com/releases/2006/05/060515095600.htm


Biosensors on the nanoscale16 l.jpg

Biosensors on the Nanoscale

Nanosphere lithography (NSL) derived triangular Ag nanoparticles were used to detect streptavidin down to one picomolar concentrations.

http://www.chem.northwestern.edu/~vanduyne/pdf/MRS_723_O311-0316_2002.pdf

Current Research

http://faculty.washington.edu/stenkamp/stefanieweb/hydro.html


Biosensors on the nanoscale17 l.jpg

Biosensors on the Nanoscale

The School of Biomedical Engineering has developed an anti-body based piezoelectric nanobiosensor to be used for anthrax,HIV hepatitis detection.

Current Research

http://www.biomed.drexel.edu/TechPort/Contents/Lec/Overview/Lec_BioSensors.pdf


Potential applications l.jpg

Potential Applications

  • Clinical diagnostics

  • Food and agricultural processes

  • Environmental (air, soil, and water) monitoring

  • Detection of warfare agents.

www.fuji-keizai.com/e/report/biosensor2004_e.html


Conclusion l.jpg

Conclusion

The possibilities are endless!

http://www.must.edu.my/~ccyeo/topics/biosensor.jpg


  • Login