Ion chromatographic determination of small concentrations of nitrate in solutions of high salinity
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Ion-chromatographic determination of small concentrations of nitrate in solutions of high salinity. Michael Raessler and Ines Hilke Max-Planck-Institut fuer Biogeochemie, Hans-Knoell-Strasse 10, D-07745 Jena, Germany. Common methods for the determination of small amounts of nitrate.

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Ion-chromatographic determination of small concentrations of nitrate in solutions of high salinity

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Ion chromatographic determination of small concentrations of nitrate in solutions of high salinity

Ion-chromatographic determination of small concentrations of nitrate in solutions of high salinity

Michael Raessler and Ines Hilke

Max-Planck-Institut fuer Biogeochemie, Hans-Knoell-Strasse 10, D-07745 Jena, Germany


Common methods for the determination of small amounts of nitrate

Common methods for the determination of small amounts of nitrate

Capillary electrophoresis

Chemilumnescence

Electrochemical detection

Fluorescence

Disadvantage: not appropriate to solutions of high sanility


Ion chromatography with uv detection instrumental set up

Ion Chromatography with UV Detection Instrumental Set-up

Instrument:Dionex 500

Pump module:Dionex GP 40

Detection:Dionex AD 20 (UV/VIS detector)

Sample loop:100 µl

Column: Hamilton PRP X-100, 250 x 4,1 mm

Eluent: 0,1 M KCl based on KCl 99,999% „suprapur“ (Merck)

Flowrate:2 mL/min

Wavelength:λ = 210 nm


Ion chromatographic determination of small concentrations of nitrate in solutions of high salinity

Application:Determination of NO3- in soil extracts of 1 M KCl

Problem:KCl of „p.A.“ grade may contain up to 0,005 % Br-

 Solution of 1 M CKl contains up to 1725 µg/L Br-

 Br- interferes with NO3- determination at trace level


1500 g l no 3 in 1 m kcl of p a grade

1500 µg/L NO3- in 1 M KCl of „p.A.“ grade


150 g l no 3 in 1 m kcl of p a grade

150 µg/L NO3- in 1 M KCl of „p.A.“ grade


Reliable determination of no 3 if concentration 450 g l

Reliable determination of NO3-,if concentration ≥ 450µg/L

Solution: Spiking

50µL of spike containing 50mg/L NO3- are added to 5 mL of extract

Correction factor necessary:  Volume of extract is changed by 1%!


Ion chromatographic determination of small concentrations of nitrate in solutions of high salinity

Concentration after additionofspike [µg/L]: 631,1

Mean (N=10) [µg/L]:629,4±7,8

Recovery [%] :99,7%

RSD [%]:1,24


Chromatogram of soil extract b 1 523 g l no 3

Chromatogram of soil extract „B-1“ (523 µg/L NO3-)


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