Ion chromatographic determination of small concentrations of nitrate in solutions of high salinity
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Ion-chromatographic determination of small concentrations of nitrate in solutions of high salinity. Michael Raessler and Ines Hilke Max-Planck-Institut fuer Biogeochemie, Hans-Knoell-Strasse 10, D-07745 Jena, Germany. Common methods for the determination of small amounts of nitrate.

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Ion chromatographic determination of small concentrations of nitrate in solutions of high salinity

Ion-chromatographic determination of small concentrations of nitrate in solutions of high salinity

Michael Raessler and Ines Hilke

Max-Planck-Institut fuer Biogeochemie, Hans-Knoell-Strasse 10, D-07745 Jena, Germany


Common methods for the determination of small amounts of nitrate
Common methods for the determination of small amounts of nitrate

Capillary electrophoresis

Chemilumnescence

Electrochemical detection

Fluorescence

Disadvantage: not appropriate to solutions of high sanility


Ion chromatography with uv detection instrumental set up
Ion Chromatography with UV Detection Instrumental Set-up nitrate

Instrument: Dionex 500

Pump module: Dionex GP 40

Detection: Dionex AD 20 (UV/VIS detector)

Sample loop: 100 µl

Column: Hamilton PRP X-100, 250 x 4,1 mm

Eluent: 0,1 M KCl based on KCl 99,999% „suprapur“ (Merck)

Flowrate: 2 mL/min

Wavelength: λ = 210 nm


Application: nitrateDetermination of NO3- in soil extracts of 1 M KCl

Problem:KCl of „p.A.“ grade may contain up to 0,005 % Br-

 Solution of 1 M CKl contains up to 1725 µg/L Br-

 Br- interferes with NO3- determination at trace level


1500 g l no 3 in 1 m kcl of p a grade
1500 µg/L NO nitrate3- in 1 M KCl of „p.A.“ grade


150 g l no 3 in 1 m kcl of p a grade
150 µg/L NO nitrate3- in 1 M KCl of „p.A.“ grade


Reliable determination of no 3 if concentration 450 g l
Reliable determination of NO nitrate3-,if concentration ≥ 450µg/L

Solution: Spiking

50µL of spike containing 50mg/L NO3- are added to 5 mL of extract

Correction factor necessary:  Volume of extract is changed by 1%!


Concentration after addition nitrateofspike [µg/L]: 631,1

Mean (N=10) [µg/L]: 629,4±7,8

Recovery [%] : 99,7%

RSD [%]: 1,24



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