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银杏雌雄株性别鉴别的研究进展 PowerPoint PPT Presentation


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银杏雌雄株性别鉴别的研究进展. 程勤贤 2004.12.21. 银杏雌雄异株 , 实生苗定植后一般需要 20 ~ 30 年才能开花结果 , 分出雌雄。由于银杏种子在成熟时具有一种特殊的臭味 , 因此 , 城市绿化需要雄株 , 而以生产白果为目的的银杏栽培主要需要雌株。因此银杏实生幼苗的性别早期鉴定 , 不仅具有理论意义 , 同时也具有实用价值。国内外学者曾在其形态学、生理生化指标、同工酶谱、染色体核型和分子标记鉴定等方面进行了研究 , 为进一步研究、开发和利用银杏这一珍贵资源提供依据和参考 。. 1. 形态学鉴定研究. 外观形态. 雄株. 雌株. 种籽.

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银杏雌雄株性别鉴别的研究进展

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2004.12.21


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,12 :CATPOD

19;29;34;44


,,320%;,,,

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,345 ,15.19%20.88%,41.91%52.92%,,,


3

:mol(CO2)/(m2s)

4

:mmol(H2O)/(m2s)

5


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3.

  • ,Tanaka,18,Newcomer,,,C.L.Lee()Pollock,,4,31,1,1,,,,,


,,4,3,,10,2n=2x=24=4m(2sat)+8sm(2sat)+12st


1214891010 356711122.073B6.72%~13.88%


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  • 42,,,,12,,9,2,,


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RFLP(Restriction Fragment Length Polymorphism )

(), RAPD(Random Amplified Polymorphic DNAs)

AFLP(Amplified Fragment Length Polymorphism)

SSR(Simple Sequence Repeats)

ISSR(Inter Simple Sequence Repeat)

SNP(Single Nucleotide Polymorphism)


RAPDRAPD300200DNA99.98%1RAPDTGATCCCTGG

RAPD682bp

,1,Sau3A,DNASau3A,2PCR,300-3000bp2PCR,75000DNA66,500-2000bp,800bp1


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